In this work, we develop methodologies for analyzing and cross comparing metabolic models. We investigate three important metabolic networks to discuss the complexity of biological organization of organisms, modeling and system properties. In particular, we analyze these metabolic networks because of their biotechnological and basic science importance: the Photosynthetic Carbon Metabolism in a general leaf, the Rhodobacter spheroides bacterium, and the Chlamydomonas reinhardtii alga. We adopt single and multi-objective optimization algorithms to maximize the CO2 uptake rate and the production of metabolites of industrial interest, or for ecological purposes. We focus both on the level of genes (e.g., finding genetic manipulations to increase the production of one or more metabolites), and on finding concentration enzymes for improving the CO2 consumption. We find that R. spheroides is able to absorb an amount of CO2 until 57.452 mmolh−1 gDW−1 , while C. reinhardtii obtains a maximum of 6.7331. We report that the Pareto front analysis proves extremely useful to compare different organisms, as well as providing the possibility to investigate them with the same framework. By using the sensitivity and robustness analysis, our framework identifies the most sensitive and fragile components of the biological systems we take into account, allowing us to compare their models. We adopt the identifiability analysis to detect functional relations among enzymes: we observe that RuBisCO, GAPDH and FBPase belong to the same functional group, as suggested also by the sensitivity analysis.

Efficient Behavior of Photosynthetic Organelles via Pareto Optimality, Identifiability and Sensitivity Analysis / Giovanni Carapezza;Renato Umeton;Jole Costanza;Claudio Angione;Giovanni Stracquadanio;Alessio Papini;Pietro Lio;Giuseppe Nicosia. - In: ACS SYNTHETIC BIOLOGY. - ISSN 2161-5063. - STAMPA. - 2(5):(2013), pp. 274-278. [10.1021/sb300102k]

Efficient Behavior of Photosynthetic Organelles via Pareto Optimality, Identifiability and Sensitivity Analysis

PAPINI, ALESSIO;
2013

Abstract

In this work, we develop methodologies for analyzing and cross comparing metabolic models. We investigate three important metabolic networks to discuss the complexity of biological organization of organisms, modeling and system properties. In particular, we analyze these metabolic networks because of their biotechnological and basic science importance: the Photosynthetic Carbon Metabolism in a general leaf, the Rhodobacter spheroides bacterium, and the Chlamydomonas reinhardtii alga. We adopt single and multi-objective optimization algorithms to maximize the CO2 uptake rate and the production of metabolites of industrial interest, or for ecological purposes. We focus both on the level of genes (e.g., finding genetic manipulations to increase the production of one or more metabolites), and on finding concentration enzymes for improving the CO2 consumption. We find that R. spheroides is able to absorb an amount of CO2 until 57.452 mmolh−1 gDW−1 , while C. reinhardtii obtains a maximum of 6.7331. We report that the Pareto front analysis proves extremely useful to compare different organisms, as well as providing the possibility to investigate them with the same framework. By using the sensitivity and robustness analysis, our framework identifies the most sensitive and fragile components of the biological systems we take into account, allowing us to compare their models. We adopt the identifiability analysis to detect functional relations among enzymes: we observe that RuBisCO, GAPDH and FBPase belong to the same functional group, as suggested also by the sensitivity analysis.
2013
2(5)
274
278
Giovanni Carapezza;Renato Umeton;Jole Costanza;Claudio Angione;Giovanni Stracquadanio;Alessio Papini;Pietro Lio;Giuseppe Nicosia
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/787365
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