Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
Whispering Gallery Mode Aptasensors for Detection of Blood Proteins / Laura Pasquardini; Simone Berneschi; Andrea Barucci; Franco Cosi; Ramona Dallapiccola; Massimiliano Insinna; Lorenzo Lunelli; Gualtiero Nunzi Conti; Cecilia Pederzolli; Simone Salvadori; Silvia Soria. - In: JOURNAL OF BIOPHOTONICS. - ISSN 1864-063X. - ELETTRONICO. - 6:(2013), pp. 178-187. [10.1002/jbio.201200013]
Whispering Gallery Mode Aptasensors for Detection of Blood Proteins
INSINNA, MASSIMILIANO;SALVADORI, SIMONE;
2013
Abstract
Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system.
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