The Burkholderia cenocepacia J2315 is Gram-negative bacterium that is a pathogen for cystic fibrosis (CF) patients. It displays a high-level of resistance to most antimicrobial drugs. In Gram-negative bacteria, the Resistance-Nodulation-Cell Division (RND) transporter family has a poorly understood role in multidrug resistance. In a previous publication we analysed the RND-4 and RND-9 transporters by microarray analysis. The obtained results suggested that only RND-4 contributes to the antibiotic resistance. The aim of this study was to investigate the role of this efflux transporter from a proteomic point of view. Methods: We quantitatively compared the intracellular proteome of the deletion mutant B. cenocepacia J2315 impaired in RND-4 transporter (BCAL2820-22) with that of the wild type strain using two-dimensional electrophoresis. Results: The results pointed out 70 differentially expressed proteins, of which 49 were identified by mass spectrometry. We found that in RND-4 mutant strain, 13 protein spots were up-regulated whilst 35 were down-regulated. One spot was detected only in wild type J2315. Fifty percent of the 35 down-regulated proteins belong to the following functional categories: “amino acids transport and metabolism”, “nucleotides transport and metabolism”, “lipid transport and metabolism”, “translation”, “ribosomal structure and biogenesis”. Conversely, forty-six percent of 13 the up-regulated proteins belong to the following functional categories: “energy production and conversion”, “post-translational modification”, “protein turnover, chaperones”. Conclusions: These results indicate that in B. cenocepacia J2315 the RND- 4 gene deletion affects, directly or indirectly, some traits of cell physiology, suggesting for this transporter a wider role than just in drug resistance.

RND-4 efflux transporter gene deletion in Burkholderia cenocepacia J2315: a proteomic analysis / T. Gamberi; S. Rocchiccioli; M. C. Papaleo; F. Magherini; L. Citti ; S. Buroni; S. Bazzini; C. Udine; E. Perrin; A. Modesti; R. Fani. - In: JOURNAL OF PROTEOME SCIENCE AND COMPUTATIONAL BIOLOGY. - ISSN 2050-2273. - ELETTRONICO. - (2013), pp. 1-14.

RND-4 efflux transporter gene deletion in Burkholderia cenocepacia J2315: a proteomic analysis

GAMBERI, TANIA;PAPALEO, MARIA CRISTIANA;MAGHERINI, FRANCESCA;PERRIN, ELENA;MODESTI, ALESSANDRA;FANI, RENATO
2013

Abstract

The Burkholderia cenocepacia J2315 is Gram-negative bacterium that is a pathogen for cystic fibrosis (CF) patients. It displays a high-level of resistance to most antimicrobial drugs. In Gram-negative bacteria, the Resistance-Nodulation-Cell Division (RND) transporter family has a poorly understood role in multidrug resistance. In a previous publication we analysed the RND-4 and RND-9 transporters by microarray analysis. The obtained results suggested that only RND-4 contributes to the antibiotic resistance. The aim of this study was to investigate the role of this efflux transporter from a proteomic point of view. Methods: We quantitatively compared the intracellular proteome of the deletion mutant B. cenocepacia J2315 impaired in RND-4 transporter (BCAL2820-22) with that of the wild type strain using two-dimensional electrophoresis. Results: The results pointed out 70 differentially expressed proteins, of which 49 were identified by mass spectrometry. We found that in RND-4 mutant strain, 13 protein spots were up-regulated whilst 35 were down-regulated. One spot was detected only in wild type J2315. Fifty percent of the 35 down-regulated proteins belong to the following functional categories: “amino acids transport and metabolism”, “nucleotides transport and metabolism”, “lipid transport and metabolism”, “translation”, “ribosomal structure and biogenesis”. Conversely, forty-six percent of 13 the up-regulated proteins belong to the following functional categories: “energy production and conversion”, “post-translational modification”, “protein turnover, chaperones”. Conclusions: These results indicate that in B. cenocepacia J2315 the RND- 4 gene deletion affects, directly or indirectly, some traits of cell physiology, suggesting for this transporter a wider role than just in drug resistance.
2013
1
14
T. Gamberi; S. Rocchiccioli; M. C. Papaleo; F. Magherini; L. Citti ; S. Buroni; S. Bazzini; C. Udine; E. Perrin; A. Modesti; R. Fani
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/799667
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