The thermal transitions of fibrillar collagen are investigated with second-harmonic generation polarization anisotropy microscopy. Second-harmonic generation images and polarization anisotropy profiles of corneal stroma heated in the 35-80°C range are analyzed by means of a theoretical model that is suitable to probe principal intramolecular and interfibrillar parameters of immediate physiological interest.
Thermal Transitions of Fibrillar Collagen Unveiled by Second-Harmonic Generation Microscopy of Corneal Stroma / Paolo Matteini;Riccardo Cicchi;Fulvio Ratto;Dimitrios Kapsokalyvas;Francesca Rossi;Marella de Angelis;Francesco S. Pavone;Roberto Pini. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 103:(2012), pp. 1179-1187. [10.1016/j.bpj.2012.07.055]
Thermal Transitions of Fibrillar Collagen Unveiled by Second-Harmonic Generation Microscopy of Corneal Stroma
CICCHI, RICCARDO;PAVONE, FRANCESCO SAVERIO;
2012
Abstract
The thermal transitions of fibrillar collagen are investigated with second-harmonic generation polarization anisotropy microscopy. Second-harmonic generation images and polarization anisotropy profiles of corneal stroma heated in the 35-80°C range are analyzed by means of a theoretical model that is suitable to probe principal intramolecular and interfibrillar parameters of immediate physiological interest.
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