The genus Burkholderia includes a variety of species, with opportunistic human pathogenic strains, whose increasing resistance to antibiotics has become a public health problem. A major role in multi-drug resistance could be played by multidrug efflux pumps belonging to the RND superfamily, an ubiquitous group of proteins that appears to be mainly involved in antibiotic resistance in Gram− bacteria. Objectives and Methods: We performed a deep analysis of the distribution of representatives of all the 8 families belonging to this superfamily in 26 Burkholderia completely sequenced genomes, to evaluate their possible use as a target for novel antimicrobial drugs. A total of 417 putative RND proteins were identified. Through different in silico analyses, most of these sequences were characterized and a core of proteins conserved in all Burkholderia genomes was identified. Conclusion: The whole body of data obtained on the presence and distribution of RND proteins in Burkholderia genus shed some light on both the physiological role(s) played by these proteins and their evolution in this genus; besides, the core of proteins identified might serve as a basis for experimental tests, already in progress, aimed at checking their possible use as novel targets in antimicrobial therapy against Burkholderia species. Lastly, the analyses performed allowed to refine the genome annotation of the genomes analyzed.

Searching for new antimicrobial targets in Burkholderia genus: In silico analysis of the RND superfamily / E. Perrin; M. Fondi; M.C. Papaleo; I. Maida; G. Emiliani; S. Buroni; M.R. Pasca; G. Riccardi; R. Fani. - In: JOURNAL OF CYSTIC FIBROSIS. - ISSN 1569-1993. - STAMPA. - (2013), pp. S73-S73.

Searching for new antimicrobial targets in Burkholderia genus: In silico analysis of the RND superfamily

PERRIN, ELENA;FONDI, MARCO;PAPALEO, MARIA CRISTIANA;MAIDA, ISABEL;EMILIANI, GIOVANNI;FANI, RENATO
2013

Abstract

The genus Burkholderia includes a variety of species, with opportunistic human pathogenic strains, whose increasing resistance to antibiotics has become a public health problem. A major role in multi-drug resistance could be played by multidrug efflux pumps belonging to the RND superfamily, an ubiquitous group of proteins that appears to be mainly involved in antibiotic resistance in Gram− bacteria. Objectives and Methods: We performed a deep analysis of the distribution of representatives of all the 8 families belonging to this superfamily in 26 Burkholderia completely sequenced genomes, to evaluate their possible use as a target for novel antimicrobial drugs. A total of 417 putative RND proteins were identified. Through different in silico analyses, most of these sequences were characterized and a core of proteins conserved in all Burkholderia genomes was identified. Conclusion: The whole body of data obtained on the presence and distribution of RND proteins in Burkholderia genus shed some light on both the physiological role(s) played by these proteins and their evolution in this genus; besides, the core of proteins identified might serve as a basis for experimental tests, already in progress, aimed at checking their possible use as novel targets in antimicrobial therapy against Burkholderia species. Lastly, the analyses performed allowed to refine the genome annotation of the genomes analyzed.
2013
E. Perrin; M. Fondi; M.C. Papaleo; I. Maida; G. Emiliani; S. Buroni; M.R. Pasca; G. Riccardi; R. Fani
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/809073
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