Human polyomavirus JC (JCPyV) reactivation causing progressive multifocal leukoencephalopathy is a main concern under immunosuppressive conditions. Recently, it has been reported that JCPyV microRNAs down-regulating early viral expression and targeting host factors may aid the virus to escape immune elimination. Here the JCPyV 5p and 3p microRNA expression was investigated in vitro and ex vivo using microRNA quantitative technologies. In vitro, the microRNA expression was checked during the infection of kidney-derived SV40 transformed COS-7 cells and hematopoietic progenitor KG-1 cells with a JCPyV molecular clone. Both microRNA expression was observed from 24 hours after the infection into the cells as well as in the exosomes obtained from the supernatant of infected cells. However, despite JCPyV DNA positivity was observed in both cellular substrates, an efficient production of progeny virus was obtained only with COS-7 cells. Ex vivo, the JCPyV 5p and 3p microRNA expression was investigated in peripheral mononuclear blood cells (PBMC) of multiple sclerosis patients treated with natalizumab and showing JCPyV asymptomatic presence in blood. In this context, whereas the 5p showed higher prevalence than that of 3p, the two microRNA expression was observed in all the PBMC DNA positive patients. Of note, in samples with PBMC and plasma viral DNA double positivity the microRNA expression was lower than in samples with PBMC positivity only. These preliminary finding suggest that future investigation of host and viral factors that may modulate these JCPyV microRNA expression playing a role in viral reactivation are required.
|Titolo:||In vitro and ex vivo detection of human polyomavirus JC microRNA|
|Anno di registrazione:||2013|
|Autori di Ateneo:|
|Autori:||Simone Giannecchini; Valeria Clausi; Irene Giovannelli; Anna Repice; Luca Massacesi; Alberta Azzi|
|Appare nelle tipologie:||1c - Abstract su rivista|
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|Giannecchini et al. 2013.pdf||Altro||DRM non definito||Administrator|