We describe novel implementations of light-sheet microscopy to resolve neuronal anatomy in whole fixed brains with cellular resolution. Moving to living samples, we show how real-time dynamics of brain rewiring can be visualized through two-photon microscopy with the spatial resolution of single synaptic contacts. The plasticity of the injured brain can also be dissected through cutting-edge optical methods that specifically ablate single neuronal processes.
ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION / L. SILVESTRI;A. L. ALLEGRA MASCARO;J. LOTTI;L. SACCONI;F. S. PAVONE. - In: JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES. - ISSN 1793-5458. - STAMPA. - 06:(2013), pp. 1230002-1230002. [10.1142/S1793545812300029]
ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION
SILVESTRI, LUDOVICO;ALLEGRA MASCARO, ANNA LETIZIA;SACCONI, LEONARDO;PAVONE, FRANCESCO SAVERIO
2013
Abstract
We describe novel implementations of light-sheet microscopy to resolve neuronal anatomy in whole fixed brains with cellular resolution. Moving to living samples, we show how real-time dynamics of brain rewiring can be visualized through two-photon microscopy with the spatial resolution of single synaptic contacts. The plasticity of the injured brain can also be dissected through cutting-edge optical methods that specifically ablate single neuronal processes.
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