The genus Burkholderia includes a variety of species, with opportunistic human pathogenic strains, whose increasing resistance to antibiotics has become a public health problem. A major role in multi-drug resistance might be played by multidrug efflux pumps belonging to the RND superfamily, an ubiquitous group of proteins that appears to be mainly involved in antibiotic resistance in Gram- bacteria. In the first part of our work, we performed a deep bioinformatics analysis of the distribution of representatives of all the 8 families belonging to this superfamily in 26 Burkholderia completely sequenced genomes. A total of 417 putative RND proteins were identified. Through different in silico analyses, most of these sequences were characterized and a core of proteins conserved in all Burkholderia genomes was identified. We focused the attention on the pair of proteins SecD-F belonging to the core, because they are highly conserved in all the genomes analyzed, suggesting that they can play an important role in these bacteria; moreover, they are involved in antibiotic resistance and in the transport of some virulence factors in other microorganisms. The production and characterization of deletion mutants of these proteins in the model system B. cenocepacia J2315 is in progress, to understand their role in this bacteria and to check their possible use as novel targets in antimicrobial therapy against Burkholderia species.

Searching for new antimicrobial targets in the Burkholderia genus: from in silico analysis to experimental characterization / E. Perrin; M. Fondi; I. Maida; S. Buroni; M.R. Pasca; G. Riccardi; R. Fani. - STAMPA. - (2013), pp. 37-37. (Intervento presentato al convegno Microbiology 2013 tenutosi a Ischia, Italy nel 19-21 settembre).

Searching for new antimicrobial targets in the Burkholderia genus: from in silico analysis to experimental characterization

PERRIN, ELENA;FONDI, MARCO;MAIDA, ISABEL;FANI, RENATO
2013

Abstract

The genus Burkholderia includes a variety of species, with opportunistic human pathogenic strains, whose increasing resistance to antibiotics has become a public health problem. A major role in multi-drug resistance might be played by multidrug efflux pumps belonging to the RND superfamily, an ubiquitous group of proteins that appears to be mainly involved in antibiotic resistance in Gram- bacteria. In the first part of our work, we performed a deep bioinformatics analysis of the distribution of representatives of all the 8 families belonging to this superfamily in 26 Burkholderia completely sequenced genomes. A total of 417 putative RND proteins were identified. Through different in silico analyses, most of these sequences were characterized and a core of proteins conserved in all Burkholderia genomes was identified. We focused the attention on the pair of proteins SecD-F belonging to the core, because they are highly conserved in all the genomes analyzed, suggesting that they can play an important role in these bacteria; moreover, they are involved in antibiotic resistance and in the transport of some virulence factors in other microorganisms. The production and characterization of deletion mutants of these proteins in the model system B. cenocepacia J2315 is in progress, to understand their role in this bacteria and to check their possible use as novel targets in antimicrobial therapy against Burkholderia species.
2013
Microbiology 2013
Microbiology 2013
Ischia, Italy
E. Perrin; M. Fondi; I. Maida; S. Buroni; M.R. Pasca; G. Riccardi; R. Fani
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/820354
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