We describe a microscopy technique that, combining wide-field single molecule microscopy, bifocal imaging and Highly Inclined and Laminated Optical sheet (HILO) microscopy, allows a 3D tracking with nanometer accuracy of single fluorescent molecules in vitro and in living cells.
Techniques for 3D tracking of single molecules with nanometer accuracy in living cells / L. Gardini;M. Capitanio;F. S. Pavone. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - STAMPA. - 8797:(2013), pp. 879703-1-879703-7. (Intervento presentato al convegno Advanced Microscopy Techniques III) [10.1117/12.2032502].
Techniques for 3D tracking of single molecules with nanometer accuracy in living cells
GARDINI, LUCIA;CAPITANIO, MARCO;PAVONE, FRANCESCO SAVERIO
2013
Abstract
We describe a microscopy technique that, combining wide-field single molecule microscopy, bifocal imaging and Highly Inclined and Laminated Optical sheet (HILO) microscopy, allows a 3D tracking with nanometer accuracy of single fluorescent molecules in vitro and in living cells.
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