Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.

SSNMR of biosilica-entrapped enzymes permits an easy assessment of preservation of native conformation in atomic detail / Marco Fragai;Claudio Luchinat;Tommaso Martelli;Enrico Ravera;Irit Sagi;Inna Solomonov;Yael Udi. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - STAMPA. - 50:(2014), pp. 421-423. [10.1039/c3cc46896h]

SSNMR of biosilica-entrapped enzymes permits an easy assessment of preservation of native conformation in atomic detail

FRAGAI, MARCO;LUCHINAT, CLAUDIO;MARTELLI, TOMMASO;RAVERA, ENRICO;
2014

Abstract

Bioinspired silica coprecipitates of enzymes find a wide range of applications for analysis and catalysis in industrial and academic research. Here we used SSNMR as a proxy for the 3D structure of enzymes trapped in bioinspired silica. We show that it is easy to assess whether the enzymes retain their native conformation in atomic detail. We thus propose SSNMR as a rapid and reliable tool to analyze this kind of samples at high resolution.
2014
50
421
423
Marco Fragai;Claudio Luchinat;Tommaso Martelli;Enrico Ravera;Irit Sagi;Inna Solomonov;Yael Udi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/833303
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