Hypersaline environments are globally distributed on Earth. The effect of salinity on the sediments makes the environment suitable for peculiar microbial communities adapted to these extreme ecosystems. Bacteria are selected to survive at different salinity concentrations and halophyilic bacteria resulted to be the most common group detected in such environments. Few studies have been focused on the differences and effects of roots on microbial communities. Our study was conducted near Wuliangsuhai Lake in the western part of Inner Mongolia (Autonomous Region - China). In June 2011 we collected five samples in duplicates from hypersaline pond: WL70 and WL71, associated to the rizosphere of Phragmites australis (Cav.) Trin. ex Steud. and WL 74 of bulk soil. Microbiological analyses have been assessed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 454 pyrosequencing. Cluster analysis based on the ARISA of the bacterial communities showed high similarity of the duplicates within the same sample site. The rhizobacterial communities clustered differently with respect to the samples of bulk soil. In the root-free soil, 454 pyrosequencing data showed that Cyanobacteria, Deferribacteres, Opitutae resulted to be most abundant taxa or to be at least present. On the other hand, Deinococci were present only in the rhizosphere samples. The other classes were present both in the bulk soil sample and in the rhizosphere sample. Consequently, here we show that the peculiar rhizosphere environment, rather than saline stress, is a stronger factor determining the overall genetic structure and taxonomic diversity of dwelling bacterial community.
Rhizobacterial communities of Phragmites australis grown in hypersaline ponds / L. Borruso; G. Bacci; A. Mengoni; R. De Philippis; L. Brusetti. - STAMPA. - (2013), pp. 370-370. (Intervento presentato al convegno Second International Conference on Microbial Diversity 2013- Microbial Interaction in Complex ecosystems tenutosi a Torino, Italia nel 23-25.10.2013).