Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20-C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-D-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola / Baroni F; Pazzagli L; Luti S; Scala A; Martellini F; Franzoni L; Pertinhez TA; Spisni A. - In: BIOMOLECULAR NMR ASSIGNMENTS. - ISSN 1874-2718. - ELETTRONICO. - (2013), pp. 1-5. [10.1007/s12104-013-9527-x]
1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola.
PAZZAGLI, LUIGIA;LUTI, SIMONE;SCALA, ANIELLO;MARTELLINI, FEDERICA;
2013
Abstract
Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20-C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-D-glucosamine) interaction, in Pop1 is predicted to be fully disordered.File | Dimensione | Formato | |
---|---|---|---|
2013- cerato-populin assignment.pdf
Accesso chiuso
Tipologia:
Altro
Licenza:
Open Access
Dimensione
503.62 kB
Formato
Adobe PDF
|
503.62 kB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.