Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20-C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-D-glucosamine) interaction, in Pop1 is predicted to be fully disordered.

1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola / Baroni F; Pazzagli L; Luti S; Scala A; Martellini F; Franzoni L; Pertinhez TA; Spisni A. - In: BIOMOLECULAR NMR ASSIGNMENTS. - ISSN 1874-2718. - ELETTRONICO. - (2013), pp. 1-5. [10.1007/s12104-013-9527-x]

1H, 15N and 13C resonance assignment of cerato-populin, a fungal PAMP from Ceratocystis populicola.

PAZZAGLI, LUIGIA;LUTI, SIMONE;SCALA, ANIELLO;MARTELLINI, FEDERICA;
2013

Abstract

Plant pathogenic fungi secrete several non-catalytic proteins involved in various aspects of the pathogenesis process. Amongst these, cerato-populin (Pop1) produced by Ceratocystis populicola; a protein orthologous of cerato-platanin (CP), the core member of the CP family. These two proteins interact with host and non-host plants. In plane leaves they induce synthesis of phytoalexins, disruption of intercellular and intracellular leaf tissue, cell plasmolysis, programmed cell death, over-expression of defence-related genes, H2O2 and NO production, activation of MAPK cascade and plant resistance. All these features point to CP and Pop1 as defence inducers, though Pop1 shows a reduced efficiency. Pop1/CP similarity is 73 %. CD spectroscopy highlights some secondary structure differences between Pop1 and CP. Indeed, the region between the first two cysteines (C20-C57), that in CP includes the β2-strand and it is involved in GlcNAc (N-acetyl-D-glucosamine) interaction, in Pop1 is predicted to be fully disordered.
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Baroni F; Pazzagli L; Luti S; Scala A; Martellini F; Franzoni L; Pertinhez TA; Spisni A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/853498
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