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|Titolo:||Genome scale metabolic reconstruction and constraints-based modelling of the Antarctic strain Pseudoalteromonas haloplanktis TAC125|
|Autori interni:||FONDI, MARCO|
|Data di pubblicazione:||2015|
|Abstract:||The Antarctic strain Pseudoalteromonas haloplanktis TAC125 is one of the model organisms of cold-adapted bacteria and is currently exploited as a new alternative expression host for numerous biotechnological applications. Here, we investigated several metabolic features of this strain through in silico modelling and functional integration of –omics data. A genome-scale metabolic model of P. haloplanktis TAC125 was reconstructed, encompassing information on 721 genes, 1133 metabolites and 1322 reactions. The predictive potential of this model was validated against a set of experimentally determined growth rates and a large dataset of growth phenotypic data. Furthermore, evidence synthesis from proteomics, phenomics, physiology and metabolic modeling data revealed possible drawbacks of cold shock-dependent changes in gene expression on the overall metabolic network of P. haloplanktis TAC125. These included, for example, variations in its central metabolism, amino acids degradation and fatty acids biosynthesis. The one described here is the first genome scale metabolic model of an Antarctic microbial strain reconstructed so far. It allowed a system-level investigation of variations in cellular metabolic fluxes following a cold shock. It represents a valuable platform for further investigations on P. haloplanktis TAC125 cellular functional states and for the design of more focused strategies for its possible biotechnological exploitation.|
|Appare nelle tipologie:||1a - Articolo su rivista|
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