Members of the genus Geosmithia (Ascomycota:Hypocreales) are mitosporic filamentous fungi distribuited worldwide and living in the galleries drilled by phloeophagous insects in broad-leaved trees. Previous work had shown that the species Geosmithia pallida harboured a gene encoding the class II hydrophobin GEO1. In order to analyze the variability of the geo1 gene in the genus Geosmithia, we investigated the presence of its sequence in 35 species representing the whole genetic diversity of the genus and derived from different host plants and geographic locations. The geo1 gene was detected in 26 species where it maintained the general organization shown in G. pallida, with three exons and two introns. Size variations were found in both introns and in the first exon, the latter being due to the presence of a microsatellite which corresponded to a stretch of glycine residues in the deduced proteins. At the amino acid level the deduced proteins had 44.6% homology and no major differences were found in the biochemical parameters (pI, GRAVY index, hydropathy plots). Finally, GEO1 production in the fungal culture filtrate was tested by turbidimetric assay and Western blotting. Phylogenetic analysis based on the geo1 sequences did not correspond to the multigene tree generated from neutral markers, thus suggesting that sequence similarities could be influenced by factors other than phylogenetic relatedness.
|Titolo:||Interspecific variability of the class ii hydrophobin GEO1 in the genus Geosmithia.|
|Anno di registrazione:||2013|
|Autori di Ateneo:|
|Autori:||A. Frascella; P. P. Bettini; M. Kolarik; C. Comparini; L. Pazzagli; F. Scala; A. Scala|
|Appare nelle tipologie:||1c - Abstract su rivista|