Doxorubicin (Dox) has got a limited efficacy in the treatment of central nervous system tumors because of its poor penetration through blood-brain barrier mediated by MDR efflux transporters. We investigated the possibility that ondansetron (Ond) enhances Dox cytotoxicity in cell lines interfering with P-glycoprotein and increases Dox concentration in rat brain tissues. The MDR phenotype was studied using human hepatocellular carcinoma cell line PLC/PRF/5 (P5 and P1(0.5) clones), two subclones of NIH 3T3 cells (PSI-2 and PN1A) and two glioblastoma cell lines (A172, U87MG). Rats were pretreated with Ond before injection of Dox. Quantitative analysis of Dox was performed by mass spectrometry. Our in vitro experiments demonstrated that Ond at 10 µg/ml is not toxic to all cell lines. However, Ond reverses the MDR phenotype in P1(0.5) and PN1A cell lines. In addition, we showed that pretreatment with Ond increases Dox concentration in rat brain tissues, without increasing acute heart and renal toxicity.

Delivery of doxorubicin across the blood–brain barrier by ondansetron pretreatment: a study in vitro and in vivo / Iacopo Sardi;Ornella Fantappiè;Giancarlo la Marca;Maria Grazia Giovannini;Anna Lisa Iorio;Martina da Ros;Sabrina Malvagia;Stefania Cardellicchio;Laura Giunti;Maurizio de Martino;Roberto Mazzanti. - In: CANCER LETTERS. - ISSN 0304-3835. - ELETTRONICO. - 353:(2014), pp. 242-247. [10.1016/j.canlet.2014.07.018]

Delivery of doxorubicin across the blood–brain barrier by ondansetron pretreatment: a study in vitro and in vivo

LA MARCA, GIANCARLO;GIOVANNINI, MARIA GRAZIA;DE MARTINO, MAURIZIO;
2014

Abstract

Doxorubicin (Dox) has got a limited efficacy in the treatment of central nervous system tumors because of its poor penetration through blood-brain barrier mediated by MDR efflux transporters. We investigated the possibility that ondansetron (Ond) enhances Dox cytotoxicity in cell lines interfering with P-glycoprotein and increases Dox concentration in rat brain tissues. The MDR phenotype was studied using human hepatocellular carcinoma cell line PLC/PRF/5 (P5 and P1(0.5) clones), two subclones of NIH 3T3 cells (PSI-2 and PN1A) and two glioblastoma cell lines (A172, U87MG). Rats were pretreated with Ond before injection of Dox. Quantitative analysis of Dox was performed by mass spectrometry. Our in vitro experiments demonstrated that Ond at 10 µg/ml is not toxic to all cell lines. However, Ond reverses the MDR phenotype in P1(0.5) and PN1A cell lines. In addition, we showed that pretreatment with Ond increases Dox concentration in rat brain tissues, without increasing acute heart and renal toxicity.
2014
353
242
247
Iacopo Sardi;Ornella Fantappiè;Giancarlo la Marca;Maria Grazia Giovannini;Anna Lisa Iorio;Martina da Ros;Sabrina Malvagia;Stefania Cardellicch...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/891743
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