The aim of the present study was to relate the presence of DNA with bone preservation in order to select samples potentially suitable for ancient DNA analysis. Bones collected from several archaeological sites, different locations (cave, rockshelter or sub divo) and diachronic periods were selected for analytical and spectroscopic analysis in order to correlate bone tissue preservation with the presence of DNA. Different techniques were combined to assess the degree of preservation of organic and inorganic components. As determined by different analytical methods, preservation of the inorganic component was best associated with the presence of DNA. Conclusion: Evaluation of the bone preservation state may be an efficient step to predict the presence of DNA in ancient samples prior to aDNA analysis.
Methodological strategies to assess the degree of bone preservation for ancient DNA studies / Scorrano G.; Valentini F.; Martínez-Labarga C.; Rolfo M.F.; Fiammenghi A.; Lo Vetro D.; Martini F.; Casoli A.; Ferraris G.; Palleschi G.; Palleschi A.; Rickards O.. - In: ANNALS OF HUMAN BIOLOGY. - ISSN 0301-4460. - ELETTRONICO. - 42:(2015), pp. 10-19. [10.3109/03014460.2014.954614]
Methodological strategies to assess the degree of bone preservation for ancient DNA studies
LO VETRO, DOMENICO;MARTINI, FABIO;
2015
Abstract
The aim of the present study was to relate the presence of DNA with bone preservation in order to select samples potentially suitable for ancient DNA analysis. Bones collected from several archaeological sites, different locations (cave, rockshelter or sub divo) and diachronic periods were selected for analytical and spectroscopic analysis in order to correlate bone tissue preservation with the presence of DNA. Different techniques were combined to assess the degree of preservation of organic and inorganic components. As determined by different analytical methods, preservation of the inorganic component was best associated with the presence of DNA. Conclusion: Evaluation of the bone preservation state may be an efficient step to predict the presence of DNA in ancient samples prior to aDNA analysis.
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