In this communication, we report on the fabrication of GM1-rich solid-supported bilayer lipid membranes (ssBLM) made of sphingomyelin and cholesterol, the main components of lipid rafts,which are the physiological hosting microenvironment of GM1 on the cell membrane. The functionality of the ganglioside has been checked by measuring the apparent dissociation constant K(D) of the complex formed by the β-subunit of the cholera toxin and GM1. The value found deviates less than one order of magnitude from that measured for in vivo cells, indicating the potential of these ssBLM as optimized in vitro biomimetic platforms.

The β-subunit of cholera toxin has a high affinity for ganglioside GM1 embedded into solid supported lipid membranes with a lipid raft-like composition / Margheri G; D'Agostino R; Trigari S; Sottini S; Del Rosso M.. - In: LIPIDS. - ISSN 0024-4201. - ELETTRONICO. - 49:(2014), pp. 203-206.

The β-subunit of cholera toxin has a high affinity for ganglioside GM1 embedded into solid supported lipid membranes with a lipid raft-like composition.

D'AGOSTINO, RICCARDO;DEL ROSSO, MARIO
2014

Abstract

In this communication, we report on the fabrication of GM1-rich solid-supported bilayer lipid membranes (ssBLM) made of sphingomyelin and cholesterol, the main components of lipid rafts,which are the physiological hosting microenvironment of GM1 on the cell membrane. The functionality of the ganglioside has been checked by measuring the apparent dissociation constant K(D) of the complex formed by the β-subunit of the cholera toxin and GM1. The value found deviates less than one order of magnitude from that measured for in vivo cells, indicating the potential of these ssBLM as optimized in vitro biomimetic platforms.
2014
49
203
206
Margheri G; D'Agostino R; Trigari S; Sottini S; Del Rosso M.
File in questo prodotto:
File Dimensione Formato  
art%3A10.1007%2Fs11745-013-3845-8.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 346.18 kB
Formato Adobe PDF
346.18 kB Adobe PDF   Richiedi una copia
PDF Lipids 1.pdf

accesso aperto

Descrizione: Articolo principale
Dimensione 346.18 kB
Formato Unknown
346.18 kB Unknown

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/956370
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 19
social impact