Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.
Alkaline-Phosphatase-Based Nanostructure Assemblies for Electrochemical Detection of microRNAs / D Voccia; F Bettazzi; G Baydemir; I Palchetti. - In: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY. - ISSN 1533-4880. - STAMPA. - 15:(2015), pp. 3378-3384. [10.1166/jnn.2015.10201]
Alkaline-Phosphatase-Based Nanostructure Assemblies for Electrochemical Detection of microRNAs
PALCHETTI, ILARIA
2015
Abstract
Different nanoarchitectures, rich in enzyme labels, are herein investigated for signal amplification in the electrochemical detection of nucleic acids and in particular of microRNAs. Dendritic amplification, accomplished by the use of streptavidin and biotinylated alkaline phosphatase, and enzymedecorated liposomes are used as labels to amplify the microRNA-sensing, by their association to the probe-microRNA hybrid generated onto a gold transducer. Differential pulse voltammetry and faradaic impedance spectroscopy were employed to characterize these different amplification routes.
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