BACKGROUND: The EC-funded project SPIDIA is aimed to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for DNA molecular testing. To this purpose, a survey and a pan-European External Quality Assessment (EQA) were implemented. METHODS: SPIDIA facility sent to all the participants the same blood sample to be processed without time or temperature limitation. DNA quality parameters performed at SPIDIA facility included: UV spectrophotometric analysis of DNA purity and yield, PCR interferences study by Kineret software and DNA integrity analysis by pulsed field gel electrophoresis. RESULTS: 197 applications have been collected from 30 European countries. A high variability of DNA fragmentation was observed whereas purity, yield and PCR interferences had a narrow distribution within laboratories. A significant difference between the RNase P single copy gene quantity obtained in the DNA samples extracted with the precipitation-based method respect to those obtained with beads and column-based methods was observed. CONCLUSIONS: The results of this study will be the basis for implementing a second pan-European EQA and the results of both EQAs will be pooled and will provide the basis for the implementation of evidence-based guidelines for the pre-analytical phase of DNA analysis of blood samples

SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses / Francesca Malentacchi ;Mario Pazzagli ; Lisa Simi ;Claudio Orlando ; Ralf Wyrich ; Christina Hartmann ; Paolo Verderio ; Sara Pizzamiglio ; Chiara Maura Ciniselli; Alex Tichopad ;Michael Kubista; Stefania Gelmini. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - ELETTRONICO. - 424:(2013), pp. 274-286. [10.1016/j.cca.2013.05.012]

SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses.

MALENTACCHI, FRANCESCA;PAZZAGLI, MARIO;SIMI, LISA;ORLANDO, CLAUDIO;GELMINI, STEFANIA
2013

Abstract

BACKGROUND: The EC-funded project SPIDIA is aimed to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for DNA molecular testing. To this purpose, a survey and a pan-European External Quality Assessment (EQA) were implemented. METHODS: SPIDIA facility sent to all the participants the same blood sample to be processed without time or temperature limitation. DNA quality parameters performed at SPIDIA facility included: UV spectrophotometric analysis of DNA purity and yield, PCR interferences study by Kineret software and DNA integrity analysis by pulsed field gel electrophoresis. RESULTS: 197 applications have been collected from 30 European countries. A high variability of DNA fragmentation was observed whereas purity, yield and PCR interferences had a narrow distribution within laboratories. A significant difference between the RNase P single copy gene quantity obtained in the DNA samples extracted with the precipitation-based method respect to those obtained with beads and column-based methods was observed. CONCLUSIONS: The results of this study will be the basis for implementing a second pan-European EQA and the results of both EQAs will be pooled and will provide the basis for the implementation of evidence-based guidelines for the pre-analytical phase of DNA analysis of blood samples
2013
424
274
286
Francesca Malentacchi ;Mario Pazzagli ; Lisa Simi ;Claudio Orlando ; Ralf Wyrich ; Christina Hartmann ; Paolo Verderio ; Sara Pizzamiglio ; Chiara Maura Ciniselli; Alex Tichopad ;Michael Kubista; Stefania Gelmini
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/967997
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