ATP depletion in human platelets caused by permeabilization with saponin does not prevent serotonin secretion induced by collagen

Ruggiero, Marco; Lapetina, E. G.; Zimmerman, T. P.

Abstract: Saponin (5 to 25 micrograms/ml) produced a concentration-dependent decrease in the cellular content of total ATP and [32P]ATP in 32P-labeled human platelets. In platelets whose ATP had been profoundly decreased by saponin, Ca2+ produced phosphomonoesteratic cleavage of the polyphosphoinositides with a concomitant accumulation of phosphatidylinositol. Collagen still induced secretion of serotonin in platelets that had been treated with saponin in the presence or absence of Ca2+. This effect of collagen occurred in the absence of the formation of cyclooxygenase metabolites. In platelet permeabilized with saponin, agonist-induced secretion and aggregation seems to be unrelated to protein phosphorylation, breakdown of the inositol phospholipids by phospholipase C and formation of cyclooxygenase metabolites.

trova@unifi

Titolo:	ATP depletion in human platelets caused by permeabilization with saponin does not prevent serotonin secretion induced by collagen
Autori di Ateneo:	RUGGIERO, MARCO E. G. LAPETINA T. P. ZIMMERMAN mostra contributor esterni nascondi contributor esterni
Autori:	RUGGIERO, MARCO; E. G. LAPETINA; T. P. ZIMMERMAN
Data di pubblicazione:	1985
Rivista:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume:	131
Pagina iniziale:	620
Pagina finale:	627
Abstract:	Abstract: Saponin (5 to 25 micrograms/ml) produced a concentration-dependent decrease in the cellular content of total ATP and [32P]ATP in 32P-labeled human platelets. In platelets whose ATP had been profoundly decreased by saponin, Ca2+ produced phosphomonoesteratic cleavage of the polyphosphoinositides with a concomitant accumulation of phosphatidylinositol. Collagen still induced secretion of serotonin in platelets that had been treated with saponin in the presence or absence of Ca2+. This effect of collagen occurred in the absence of the formation of cyclooxygenase metabolites. In platelet permeabilized with saponin, agonist-induced secretion and aggregation seems to be unrelated to protein phosphorylation, breakdown of the inositol phospholipids by phospholipase C and formation of cyclooxygenase metabolites.
Handle:	http://hdl.handle.net/2158/9938
Appare nelle tipologie:	1a - Articolo su rivista

File in questo prodotto:

Non ci sono file associati a questo prodotto.

Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/2158/9938

Citazioni

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.