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EnglishAbstract: Saponin (5 to 25 micrograms/ml) produced a concentration-dependent decrease in the cellular content of total ATP and [32P]ATP in 32P-labeled human platelets. In platelets whose ATP had been profoundly decreased by saponin, Ca2+ produced phosphomonoesteratic cleavage of the polyphosphoinositides with a concomitant accumulation of phosphatidylinositol. Collagen still induced secretion of serotonin in platelets that had been treated with saponin in the presence or absence of Ca2+. This effect of collagen occurred in the absence of the formation of cyclooxygenase metabolites. In platelet permeabilized with saponin, agonist-induced secretion and aggregation seems to be unrelated to protein phosphorylation, breakdown of the inositol phospholipids by phospholipase C and formation of cyclooxygenase metabolites.
| Titolo: | ATP depletion in human platelets caused by permeabilization with saponin does not prevent serotonin secretion induced by collagen |
| Autori di Ateneo: | |
| Autori: | RUGGIERO, MARCO; E. G. LAPETINA; T. P. ZIMMERMAN |
| Data di pubblicazione: | 1985 |
| Rivista: | |
| Volume: | 131 |
| Pagina iniziale: | 620 |
| Pagina finale: | 627 |
| Abstract: | Abstract: Saponin (5 to 25 micrograms/ml) produced a concentration-dependent decrease in the cellular content of total ATP and [32P]ATP in 32P-labeled human platelets. In platelets whose ATP had been profoundly decreased by saponin, Ca2+ produced phosphomonoesteratic cleavage of the polyphosphoinositides with a concomitant accumulation of phosphatidylinositol. Collagen still induced secretion of serotonin in platelets that had been treated with saponin in the presence or absence of Ca2+. This effect of collagen occurred in the absence of the formation of cyclooxygenase metabolites. In platelet permeabilized with saponin, agonist-induced secretion and aggregation seems to be unrelated to protein phosphorylation, breakdown of the inositol phospholipids by phospholipase C and formation of cyclooxygenase metabolites. |
| Handle: | http://hdl.handle.net/2158/9938 |
| Appare nelle tipologie: | 1a - Articolo su rivista |
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