In this work, an electrochemical DNA aptasensor for acetamiprid is presented. The aptasensor is based on a dual signal amplified strategy by employing a gold-polyaniline modified screen-printed electrode as sensor platform and an enzyme-linked label for sensitive detection. The polyaniline-gold nanocomposite modified electrodes were firstly modified with a mixed monolayer of a thiol-tethered DNA aptamer and 6-mercapto-1-hexanol. The acetamiprid solution was then incubated with the aptasensor. An enzyme - Amplified detection scheme, based on the coupling of a streptavidin - Alkaline phosphatase conjugate and secondary biotinylated aptamer was then applied. The enzyme catalyzed the hydrolysis of the electroinactive 1-naphthyl-phosphate to 1-naphthol. This electroactive product was detected by means of differential pulse voltammetry. A calibration curve between 0-200 nM acetamiprid concentration range was obtained.
DNA technology for small molecules sensing: A new approach for Acetamiprid detection / Rapini, Riccardo.; Marrazza, Giovanna. - ELETTRONICO. - (2015), pp. 1-4. [10.1109/AISEM.2015.7066768]
DNA technology for small molecules sensing: A new approach for Acetamiprid detection
RAPINI, RICCARDO;MARRAZZA, GIOVANNA
2015
Abstract
In this work, an electrochemical DNA aptasensor for acetamiprid is presented. The aptasensor is based on a dual signal amplified strategy by employing a gold-polyaniline modified screen-printed electrode as sensor platform and an enzyme-linked label for sensitive detection. The polyaniline-gold nanocomposite modified electrodes were firstly modified with a mixed monolayer of a thiol-tethered DNA aptamer and 6-mercapto-1-hexanol. The acetamiprid solution was then incubated with the aptasensor. An enzyme - Amplified detection scheme, based on the coupling of a streptavidin - Alkaline phosphatase conjugate and secondary biotinylated aptamer was then applied. The enzyme catalyzed the hydrolysis of the electroinactive 1-naphthyl-phosphate to 1-naphthol. This electroactive product was detected by means of differential pulse voltammetry. A calibration curve between 0-200 nM acetamiprid concentration range was obtained.File | Dimensione | Formato | |
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