Proton-detection in solid-state NMR, enabled by high magnetic fields (>18 T) and fast magic angle spinning (>50 kHz), allows for the acquisition of traditional 1H-15N experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that 1H-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.
1 H-detected solid-state NMR of proteins entrapped in bioinspired silica: A new tool for biomaterials characterization / Ravera, Enrico; Cerofolini, Linda; Martelli, Tommaso; Louka, Alexandra; Fragai, Marco; Luchinat, Claudio. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 6:(2016), pp. 27851-27851-7. [10.1038/srep27851]
1 H-detected solid-state NMR of proteins entrapped in bioinspired silica: A new tool for biomaterials characterization
RAVERA, ENRICO;CEROFOLINI, LINDA;MARTELLI, TOMMASO;LOUKA, ALEXANDRA;FRAGAI, MARCO;LUCHINAT, CLAUDIO
2016
Abstract
Proton-detection in solid-state NMR, enabled by high magnetic fields (>18 T) and fast magic angle spinning (>50 kHz), allows for the acquisition of traditional 1H-15N experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that 1H-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.
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