This paper reports that the combination of microfluidics and dielectrophoresis (DEP) allows to culture different pancreatic ductal adenocarcinoma (PDAC) human cell lines into a cyclic olefin polymer (COP) chamber (HepaChip®), enriched by the extracellular matrix (ECM) protein collagen. We show that PDAC cells cultured into the HepaChip® (1) are vital and grow, provided they properly attach to collagen; (2) show morphological appearance and growth characteristics closer to those of cells grown as spheroids than as classical 2 dimensional (2D) in vitro cultures. Finally, preliminary experiments show that PDAC cells respond to high doses of Cisplatin perfused through the chip. Overall, the present microfluidic platform could be exploited in the future for a personalised approach to PDAC.

A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: Comparison with in vitro cultures and in vivo xenografts / Beer, Meike; Kuppalu, Nirmala; Stefanini, Matteo; Becker, Holger; Schulz, Ingo; Manoli, Sagar; Schuette, Julia; Schmees, Christian; Casazza, Armando; Stelzle, Martin; Arcangeli, Annarosa. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 7:(2017), pp. 1325-1336. [10.1038/s41598-017-01256-8]

A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: Comparison with in vitro cultures and in vivo xenografts

KUPPALU RAMAPPA, NIRMALA;STEFANINI, MATTEO;ARCANGELI, ANNAROSA
2017

Abstract

This paper reports that the combination of microfluidics and dielectrophoresis (DEP) allows to culture different pancreatic ductal adenocarcinoma (PDAC) human cell lines into a cyclic olefin polymer (COP) chamber (HepaChip®), enriched by the extracellular matrix (ECM) protein collagen. We show that PDAC cells cultured into the HepaChip® (1) are vital and grow, provided they properly attach to collagen; (2) show morphological appearance and growth characteristics closer to those of cells grown as spheroids than as classical 2 dimensional (2D) in vitro cultures. Finally, preliminary experiments show that PDAC cells respond to high doses of Cisplatin perfused through the chip. Overall, the present microfluidic platform could be exploited in the future for a personalised approach to PDAC.
2017
7
1325
1336
Goal 3: Good health and well-being for people
Beer, Meike; Kuppalu, Nirmala; Stefanini, Matteo; Becker, Holger; Schulz, Ingo; Manoli, Sagar; Schuette, Julia; Schmees, Christian; Casazza, Armando; Stelzle, Martin; Arcangeli, Annarosa
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1084967
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