A major challenge in isolating oligosaccharides from dairy streams is to enrich oligosaccharides while simultaneously reducing the content of simple sugars (mono- and disaccharides) that do not possess the desired prebiotic functions. An integrated approach based on optimized conditions that favor maximum lactose hydrolysis, monosaccharide fermentation and oligosaccharides recovery by nanofiltration was developed. Upon complete lactose hydrolysis and fermentation of the monosaccharides by yeast, nanofiltration of fermented whey permeate from colostrum enabled the recovery of 95% of the oligosaccharides at high purity. While the number of commercially available standards has limited the quantification of only a few sialylated oligosaccharides, the application of both high performance anion-exchange chromatography with pulsed amperometric detection and mass spectrometry provided a complete profile of the final product. Approximately 85% of the oligosaccharides in the final concentrate were sialylated, with the remainder being neutral.

An integrated bioprocess to recover bovine milk oligosaccharides from colostrum whey permeate / Bell, Juliana M. L. N. de Moura; Cohen, Joshua L.; Aquino, Leticia F. M. C. de; Lee, Hyeyoung; Silva, Vitor L. de Melo; Liu, Yan; Domizio, Paola; Barile, Daniela. - In: JOURNAL OF FOOD ENGINEERING. - ISSN 0260-8774. - STAMPA. - 216:(2018), pp. 27-35. [10.1016/j.jfoodeng.2017.07.022]

An integrated bioprocess to recover bovine milk oligosaccharides from colostrum whey permeate

DOMIZIO, PAOLA;
2018

Abstract

A major challenge in isolating oligosaccharides from dairy streams is to enrich oligosaccharides while simultaneously reducing the content of simple sugars (mono- and disaccharides) that do not possess the desired prebiotic functions. An integrated approach based on optimized conditions that favor maximum lactose hydrolysis, monosaccharide fermentation and oligosaccharides recovery by nanofiltration was developed. Upon complete lactose hydrolysis and fermentation of the monosaccharides by yeast, nanofiltration of fermented whey permeate from colostrum enabled the recovery of 95% of the oligosaccharides at high purity. While the number of commercially available standards has limited the quantification of only a few sialylated oligosaccharides, the application of both high performance anion-exchange chromatography with pulsed amperometric detection and mass spectrometry provided a complete profile of the final product. Approximately 85% of the oligosaccharides in the final concentrate were sialylated, with the remainder being neutral.
2018
216
27
35
Bell, Juliana M. L. N. de Moura; Cohen, Joshua L.; Aquino, Leticia F. M. C. de; Lee, Hyeyoung; Silva, Vitor L. de Melo; Liu, Yan; Domizio, Paola; Barile, Daniela
File in questo prodotto:
File Dimensione Formato  
2018_DeMoura Bell_An integrated bioprocess to recover bovine milk oligosaccharides from.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 868.67 kB
Formato Adobe PDF
868.67 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1092321
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 39
  • ???jsp.display-item.citation.isi??? 30
social impact