Cadmium (Cd) is a worldwide occupational and environmental pollutant . Cd toxicity is widely known and studied in many tissues and organs such as testis, kidney, liver, lung and brain. On the other hand, zinc (Zn) is a trace element known as coenzyme for many proteins such as methallothionein . To date, very little is known about the role of Cd and Zn in BBB permeability. To study their effects in BBB permeability in vitro, the RBE4 cell line was used and different concentrations of CdCl2 and ZnCl2 were tested. Metabolic activity (MTTassay) was performed to test the protective and preventive role of ZnCl2 on CdCl2 toxicity. Western blotting analysis was used to better investigate the molecular pathway involved in Cd-induced BBB permeability evaluating GRP78 (ER stress marker) and caspase-3 protein expression levels. Furthermore, ZO-1 and F-actin immunofluorescent staining was performed to better understand the morphological alterations and BBB permeability achieved by Cd treatment. Our preliminary data highlight the role of Cd in evoking BBB permeability by F-actin and ZO1 dislocations, triggering the caspase-3 molecular pathway activation induced by GRP78-ER stress increase. Moreover, the data clearly show how Zn is able to counteract the metabolic impairment induced by Cd treatment. Taken together these data point out the possible role of Zn in counteracting the Cd-induced BBB impairment.

Zinc protection in cadmium-induced Blood Brain Barrier permeability: a metabolic and morphological in vitro study / Branca, Jacopo J. V.; DI CESARE MANNELLI, Lorenzo; Maresca, Mario; Paternostro, Ferdinando; Sofia, Bonechi; Morucci, Gabriele; Gulisano, Massimo; Pacini, Alessandra. - In: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY. - ISSN 2038-5129. - STAMPA. - Vol. 119,:(2017), pp. 33-33. (Intervento presentato al convegno 71 th Meeting of the Italian Society of Anatomy and Histology tenutosi a Messina - Taormina nel Settembre 2017).

Zinc protection in cadmium-induced Blood Brain Barrier permeability: a metabolic and morphological in vitro study

Jacopo J. V. Branca;Lorenzo Di Cesare Mannelli;Mario Maresca;Ferdinando Paternostro;Gabriele Morucci;Massimo Gulisano;Alessandra Pacini
2017

Abstract

Cadmium (Cd) is a worldwide occupational and environmental pollutant . Cd toxicity is widely known and studied in many tissues and organs such as testis, kidney, liver, lung and brain. On the other hand, zinc (Zn) is a trace element known as coenzyme for many proteins such as methallothionein . To date, very little is known about the role of Cd and Zn in BBB permeability. To study their effects in BBB permeability in vitro, the RBE4 cell line was used and different concentrations of CdCl2 and ZnCl2 were tested. Metabolic activity (MTTassay) was performed to test the protective and preventive role of ZnCl2 on CdCl2 toxicity. Western blotting analysis was used to better investigate the molecular pathway involved in Cd-induced BBB permeability evaluating GRP78 (ER stress marker) and caspase-3 protein expression levels. Furthermore, ZO-1 and F-actin immunofluorescent staining was performed to better understand the morphological alterations and BBB permeability achieved by Cd treatment. Our preliminary data highlight the role of Cd in evoking BBB permeability by F-actin and ZO1 dislocations, triggering the caspase-3 molecular pathway activation induced by GRP78-ER stress increase. Moreover, the data clearly show how Zn is able to counteract the metabolic impairment induced by Cd treatment. Taken together these data point out the possible role of Zn in counteracting the Cd-induced BBB impairment.
2017
71 th Meeting of the Italian Society of Anatomy and Histology
71 th Meeting of the Italian Society of Anatomy and Histology
Messina - Taormina
Goal 3: Good health and well-being for people
Branca, Jacopo J. V.; DI CESARE MANNELLI, Lorenzo; Maresca, Mario; Paternostro, Ferdinando; Sofia, Bonechi; Morucci, Gabriele; Gulisano, Massimo; Paci...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1103591
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