We report on the use of organosilica shells to couple gold nanorods to functional peptides and modulate their physiochemical and biological profiles. In particular, we focus on the case of cell penetrating peptides, which are used to load tumor-tropic macrophages and implement an innovative drug delivery system for photothermal and photoacoustic applications. The presence of organosilica exerts subtle effects on multiple parameters of the particles, including their size, shape, electrokinetic potential, photostability, kinetics of endocytic uptake and cytotoxicity, which are investigated by the interplay of colorimetric methods and digital holographic microscopy. As a rule of thumb, as the thickness of organosilica increases from none to ∼30 nm, we find an improvement of the photophysical performances at the expense of a deterioration of the biological parameters. Therefore, detailed engineering of the particles for a certain application will require a careful trade-off between photophysical and biological specifications.

A multifunctional organosilica cross-linker for the bio-conjugation of gold nanorods / Lai, Sarah; Centi, Sonia; Borri, Claudia; Ratto, Fulvio; Cavigli, Lucia; Micheletti, Filippo; Kemper, Bjӧrn; Ketelhut, Steffi; Kozyreva, Tatiana; Gonnelli, Leonardo; Rossi, Francesca; Colagrande, Stefano; Pini, Roberto. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - STAMPA. - 157:(2017), pp. 174-181. [10.1016/j.colsurfb.2017.05.068]

A multifunctional organosilica cross-linker for the bio-conjugation of gold nanorods

Lai, Sarah;Centi, Sonia;Borri, Claudia;Cavigli, Lucia;Kozyreva, Tatiana;Gonnelli, Leonardo;Colagrande, Stefano;Pini, Roberto
2017

Abstract

We report on the use of organosilica shells to couple gold nanorods to functional peptides and modulate their physiochemical and biological profiles. In particular, we focus on the case of cell penetrating peptides, which are used to load tumor-tropic macrophages and implement an innovative drug delivery system for photothermal and photoacoustic applications. The presence of organosilica exerts subtle effects on multiple parameters of the particles, including their size, shape, electrokinetic potential, photostability, kinetics of endocytic uptake and cytotoxicity, which are investigated by the interplay of colorimetric methods and digital holographic microscopy. As a rule of thumb, as the thickness of organosilica increases from none to ∼30 nm, we find an improvement of the photophysical performances at the expense of a deterioration of the biological parameters. Therefore, detailed engineering of the particles for a certain application will require a careful trade-off between photophysical and biological specifications.
2017
157
174
181
Lai, Sarah; Centi, Sonia; Borri, Claudia; Ratto, Fulvio; Cavigli, Lucia; Micheletti, Filippo; Kemper, Bjӧrn; Ketelhut, Steffi; Kozyreva, Tatiana; Gonnelli, Leonardo; Rossi, Francesca; Colagrande, Stefano; Pini, Roberto
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1110056
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