Millions of premature deaths per year from cardiovascular diseases represent a global threat urging governments to increase global initiatives, as advised by World Health Organization. In particular, together with prevention and management of risk factors, the development of portable platforms for early diagnosis of cardiovascular disorders appears a fundamental task to carry out. Contemporary assays demonstrated very good accuracy for diagnosis of acute myocardial infarction (AMI), but they are based on expensive and fragile capture antibodies. Accordingly, also considering the massive demand from developing countries, we have devoted our study to an affinity-based biosensor for detection of troponin T (TnT), a preferred biomarker of AMI. This combines a stable and inexpensive molecularly imprinted polymer (MIP) based on polydopamine (PDA) with surface plasmon resonance (SPR) transduction. Herein we report the fast and specific answer upon TnT binding onto an epitope- imprinted surface that strongly encourages the further development toward antibody-free point-of-care testing for cardiac injury.

Cardiac Troponin T capture and detection in real-time via epitope-imprinted polymer and optical biosensing / Pasquale Palladino, Maria Minunni, Simona Scarano. - In: BIOSENSORS & BIOELECTRONICS. - ISSN 0956-5663. - ELETTRONICO. - 106:(2018), pp. 93-98. [10.1016/j.bios.2018.01.068]

Cardiac Troponin T capture and detection in real-time via epitope-imprinted polymer and optical biosensing

Pasquale Palladino;Maria Minunni;Simona Scarano
2018

Abstract

Millions of premature deaths per year from cardiovascular diseases represent a global threat urging governments to increase global initiatives, as advised by World Health Organization. In particular, together with prevention and management of risk factors, the development of portable platforms for early diagnosis of cardiovascular disorders appears a fundamental task to carry out. Contemporary assays demonstrated very good accuracy for diagnosis of acute myocardial infarction (AMI), but they are based on expensive and fragile capture antibodies. Accordingly, also considering the massive demand from developing countries, we have devoted our study to an affinity-based biosensor for detection of troponin T (TnT), a preferred biomarker of AMI. This combines a stable and inexpensive molecularly imprinted polymer (MIP) based on polydopamine (PDA) with surface plasmon resonance (SPR) transduction. Herein we report the fast and specific answer upon TnT binding onto an epitope- imprinted surface that strongly encourages the further development toward antibody-free point-of-care testing for cardiac injury.
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93
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Pasquale Palladino, Maria Minunni, Simona Scarano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1113760
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