OBJECTIVE: This study aimed at investigating the in vitro activity of minocycline and doxycycline on human polymorphonuclear (h-PMN) cell function. METHODS: h-PMNs were isolated from whole venous blood of healthy subjects; PMN oxidative burst was measured by monitoring ROS-induced oxidation of luminol and transendothelial migration was studied by measuring PMN migration through a monolayer of human umbilical vein endothelial cells. Differences between multiple groups were determined by ANOVA followed by Tukey's multiple comparison test; Student's t test for unpaired data for two groups. RESULTS: Minocycline (1-300 µM) concentration dependently and significantly inhibited oxidative burst of h-PMNs stimulated with 100 nM fMLP. Ten micromolar concentrations, which are superimposable to C max following a standard oral dose of minocycline, promoted a 29.8 ± 4 % inhibition of respiratory burst (P < 0.001; n = 6). Doxycycline inhibited ROS production with a lesser extent and at higher concentrations. 10-100 µM minocycline impaired PMN transendothelial migration, with maximal effect at 100 µM (42.5 ± 7 %, inhibition, n = 5, P < 0.001). CONCLUSIONS: These results added new insight into anti-inflammatory effects of minocycline exerted on innate immune h-PMN cell function.

Minocycline affects human neutrophil respiratory burst and transendothelial migration / Parenti, Astrid; Indorato, Boris; Paccosi, Sara*. - In: INFLAMMATION RESEARCH. - ISSN 1023-3830. - ELETTRONICO. - 66:(2017), pp. 107-109. [10.1007/s00011-016-0999-x]

Minocycline affects human neutrophil respiratory burst and transendothelial migration

Parenti, Astrid;Paccosi, Sara
2017

Abstract

OBJECTIVE: This study aimed at investigating the in vitro activity of minocycline and doxycycline on human polymorphonuclear (h-PMN) cell function. METHODS: h-PMNs were isolated from whole venous blood of healthy subjects; PMN oxidative burst was measured by monitoring ROS-induced oxidation of luminol and transendothelial migration was studied by measuring PMN migration through a monolayer of human umbilical vein endothelial cells. Differences between multiple groups were determined by ANOVA followed by Tukey's multiple comparison test; Student's t test for unpaired data for two groups. RESULTS: Minocycline (1-300 µM) concentration dependently and significantly inhibited oxidative burst of h-PMNs stimulated with 100 nM fMLP. Ten micromolar concentrations, which are superimposable to C max following a standard oral dose of minocycline, promoted a 29.8 ± 4 % inhibition of respiratory burst (P < 0.001; n = 6). Doxycycline inhibited ROS production with a lesser extent and at higher concentrations. 10-100 µM minocycline impaired PMN transendothelial migration, with maximal effect at 100 µM (42.5 ± 7 %, inhibition, n = 5, P < 0.001). CONCLUSIONS: These results added new insight into anti-inflammatory effects of minocycline exerted on innate immune h-PMN cell function.
2017
66
107
109
Parenti, Astrid; Indorato, Boris; Paccosi, Sara*
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1126720
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