It is well accepted that Th17 cells are a highly plastic cell subset that can be easily directed towards the Th1 phenotype in vitro and also in vivo during inflammation. However, there is an ongoing debate regarding the reverse plasticity (conversion from Th1 to Th17). We show here that ectopic ROR-γt expression can restore or initiate IL-17 expression by non-classic or classic Th1 cells, respectively, while common pro-Th17 cytokine cocktails are ineffective. This stability of the Th1 phenotype is at least partially due to the presence of a molecular machinery governed by the transcription factor Eomes, which promotes IFN-γ secretion while inhibiting the expression of ROR-γt and IL-17. By using a mouse model of T cell-dependent colitis we demonstrate that Eomes controls non-classic Th1 cell development also in vivo and promotes their pathogenic potential. Eomes expression associates to a highly inflammatory phenotype also in patients with juvenile idiopathic arthritis. Indeed, it favors the acquisition of a cytotoxic signature, and promotes the development of IFN-γ+ GM-CSF+ cells that have been described to be pathogenic in chronic inflammatory disorders. This article is protected by copyright. All rights reserved.

Eomes controls the development of Th17-derived (non-classic) Th1 cells during chronic inflammation / Mazzoni, Alessio; Maggi, Laura; Siracusa, Francesco; Ramazzotti, Matteo; Rossi, Maria Caterina; Santarlasci, Veronica; Montaini, Gianni; Capone, Manuela; Rossettini, Beatrice; De Palma, Raffaele; Kruglov, Andrey; Chang, Hyun-Dong; Cimaz, Rolando; Maggi, Enrico; Romagnani, Sergio; Liotta, Francesco; Cosmi, Lorenzo; Annunziato, Francesco. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - ELETTRONICO. - (2018), pp. 0-1. [10.1002/eji.201847677]

Eomes controls the development of Th17-derived (non-classic) Th1 cells during chronic inflammation

Mazzoni, Alessio;Maggi, Laura;Ramazzotti, Matteo;Rossi, Maria Caterina;Santarlasci, Veronica;MONTAINI, GIANNI;Capone, Manuela;ROSSETTINI, BEATRICE;Cimaz, Rolando;Maggi, Enrico;Romagnani, Sergio;Liotta, Francesco;Cosmi, Lorenzo;Annunziato, Francesco
2018

Abstract

It is well accepted that Th17 cells are a highly plastic cell subset that can be easily directed towards the Th1 phenotype in vitro and also in vivo during inflammation. However, there is an ongoing debate regarding the reverse plasticity (conversion from Th1 to Th17). We show here that ectopic ROR-γt expression can restore or initiate IL-17 expression by non-classic or classic Th1 cells, respectively, while common pro-Th17 cytokine cocktails are ineffective. This stability of the Th1 phenotype is at least partially due to the presence of a molecular machinery governed by the transcription factor Eomes, which promotes IFN-γ secretion while inhibiting the expression of ROR-γt and IL-17. By using a mouse model of T cell-dependent colitis we demonstrate that Eomes controls non-classic Th1 cell development also in vivo and promotes their pathogenic potential. Eomes expression associates to a highly inflammatory phenotype also in patients with juvenile idiopathic arthritis. Indeed, it favors the acquisition of a cytotoxic signature, and promotes the development of IFN-γ+ GM-CSF+ cells that have been described to be pathogenic in chronic inflammatory disorders. This article is protected by copyright. All rights reserved.
2018
0
1
Mazzoni, Alessio; Maggi, Laura; Siracusa, Francesco; Ramazzotti, Matteo; Rossi, Maria Caterina; Santarlasci, Veronica; Montaini, Gianni; Capone, Manuela; Rossettini, Beatrice; De Palma, Raffaele; Kruglov, Andrey; Chang, Hyun-Dong; Cimaz, Rolando; Maggi, Enrico; Romagnani, Sergio; Liotta, Francesco; Cosmi, Lorenzo; Annunziato, Francesco
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1141156
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