Comprehensive mapping and quantification of neuronal projections in the central nervous system requires high-throughput imaging of large volumes with microscopic resolution. To this end, we have developed a confocal light-sheet microscope that has been optimized for three-dimensional (3-D) imaging of structurally intact clarified whole-mount mouse brains. We describe the optical and electromechanical arrangement of the microscope and give details on the organization of the microscope management software. The software orches- trates all components of the microscope, coordinates critical timing and synchronization, and has been written in a versatile and modular structure using the LabVIEW language. It can easily be adapted and integrated to other microscope systems and has been made freely available to the light-sheet community. The tremendous amount of data routinely generated by light-sheet microscopy further requires novel strategies for data handling and storage. To complete the full imaging pipeline of our high-throughput microscope, we further elaborate on big data management from streaming of raw images up to stitching of 3-D datasets. The mesoscale neuroanat- omy imaged at micron-scale resolution in those datasets allows characterization and quantification of neuronal projections in unsectioned mouse brains

Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains / Mullenbroich M.C.; Silvestri L.; Onofri L.; Costantini I.; Van't Hoff M.; Sacconi L.; Iannello G.; Pavone F.S.. - In: NEUROPHOTONICS. - ISSN 2329-423X. - ELETTRONICO. - 2:(2015), pp. 041404-041404. [10.1117/1.NPh.2.4.041404]

Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains

Mullenbroich M. C.;Silvestri L.;Onofri L.;Costantini I.;VAN 'T HOFF, MARCEL;Pavone F. S.
2015

Abstract

Comprehensive mapping and quantification of neuronal projections in the central nervous system requires high-throughput imaging of large volumes with microscopic resolution. To this end, we have developed a confocal light-sheet microscope that has been optimized for three-dimensional (3-D) imaging of structurally intact clarified whole-mount mouse brains. We describe the optical and electromechanical arrangement of the microscope and give details on the organization of the microscope management software. The software orches- trates all components of the microscope, coordinates critical timing and synchronization, and has been written in a versatile and modular structure using the LabVIEW language. It can easily be adapted and integrated to other microscope systems and has been made freely available to the light-sheet community. The tremendous amount of data routinely generated by light-sheet microscopy further requires novel strategies for data handling and storage. To complete the full imaging pipeline of our high-throughput microscope, we further elaborate on big data management from streaming of raw images up to stitching of 3-D datasets. The mesoscale neuroanat- omy imaged at micron-scale resolution in those datasets allows characterization and quantification of neuronal projections in unsectioned mouse brains
2015
2
041404
041404
Mullenbroich M.C.; Silvestri L.; Onofri L.; Costantini I.; Van't Hoff M.; Sacconi L.; Iannello G.; Pavone F.S.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1162626
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