Purpose: To evaluate circulating endothelial and circulating progenitor cells as biomarkers in age-related macular degeneration (AMD) patients (both exudative and atrophic forms) in order to establish the possible clinical implication of their assessment. Methods: We have enrolled 44 AMD patients: 22 patients with a recently diagnosed exudative (neovascular) form (nvAMD - group A) and 22 patients with an atrophic (dry) form (drAMD – group B). The control group consisted of 22 age and sex-matched healthy subjects (group C). The number of circulating endothelial progenitor cells (EPC: CD34+/KDR+; CD133+/KDR+; CD34+/KDR+/CD133+), circulating progenitor cells (CPC: CD34+; CD133+; CD34+/CD133+) and circulating endothelial cells (CEC) were determined in the peripheral venous blood samples by flow cytometry. NvAMD patients were evaluated at baseline and 4 weeks after a loading phase of three consequent intravitreal injections of ranibizumab. Results: Comparing AMD patients with the control group, EPCs and CPCs levels were not significantly different, while AMD patients showed significantly higher levels of CECs (p=0.001). Anti-VEGF treatment with intravitreal ranibizumab was associated with a significant reduction of EPC levels, with no significant influence on CPC and CEC. Conclusion: We reported higher levels of CEC in AMD patients in comparison with the control group thereby supporting the hypothesis of an involvement of endothelial dysregulation in the AMD and a reduction of the EPC level in nvAMD patients after three intravitreal injections of ranibizumab.

Circulating endothelial and progenitor cells in age-related macular degeneration / Mucciolo, Dario Pasquale; Marcucci, Rossella; Sodi, Andrea; Cesari, Francesca; Murro, Vittoria; Rogolino, Angela; Rizzo, Stanislao; Giusti, Betti; Virgili, Gianni; Prisco, Domenico; Gori, Anna Maria. - In: EUROPEAN JOURNAL OF OPHTHALMOLOGY. - ISSN 1120-6721. - ELETTRONICO. - (2019), pp. 112067211986330-112067211986334. [10.1177/1120672119863306]

Circulating endothelial and progenitor cells in age-related macular degeneration

Mucciolo, Dario Pasquale
;
Marcucci, Rossella;Sodi, Andrea;Cesari, Francesca;Murro, Vittoria;Rogolino, Angela;Rizzo, Stanislao;Giusti, Betti;Virgili, Gianni;Prisco, Domenico;Gori, Anna Maria
2019

Abstract

Purpose: To evaluate circulating endothelial and circulating progenitor cells as biomarkers in age-related macular degeneration (AMD) patients (both exudative and atrophic forms) in order to establish the possible clinical implication of their assessment. Methods: We have enrolled 44 AMD patients: 22 patients with a recently diagnosed exudative (neovascular) form (nvAMD - group A) and 22 patients with an atrophic (dry) form (drAMD – group B). The control group consisted of 22 age and sex-matched healthy subjects (group C). The number of circulating endothelial progenitor cells (EPC: CD34+/KDR+; CD133+/KDR+; CD34+/KDR+/CD133+), circulating progenitor cells (CPC: CD34+; CD133+; CD34+/CD133+) and circulating endothelial cells (CEC) were determined in the peripheral venous blood samples by flow cytometry. NvAMD patients were evaluated at baseline and 4 weeks after a loading phase of three consequent intravitreal injections of ranibizumab. Results: Comparing AMD patients with the control group, EPCs and CPCs levels were not significantly different, while AMD patients showed significantly higher levels of CECs (p=0.001). Anti-VEGF treatment with intravitreal ranibizumab was associated with a significant reduction of EPC levels, with no significant influence on CPC and CEC. Conclusion: We reported higher levels of CEC in AMD patients in comparison with the control group thereby supporting the hypothesis of an involvement of endothelial dysregulation in the AMD and a reduction of the EPC level in nvAMD patients after three intravitreal injections of ranibizumab.
2019
112067211986330
112067211986334
Goal 3: Good health and well-being for people
Mucciolo, Dario Pasquale; Marcucci, Rossella; Sodi, Andrea; Cesari, Francesca; Murro, Vittoria; Rogolino, Angela; Rizzo, Stanislao; Giusti, Betti; Virgili, Gianni; Prisco, Domenico; Gori, Anna Maria
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1165018
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