Spectrum of ASXL1 mutations in primary myelofibrosis: Prognostic impact of the ASXL1 p.G646Wfs∗12 mutation

data reported herein suggest that care should be taken to accurately detect and report the ASXL1G646Wfs*12 variant in patients with PMF because of its strong adverse impact on OS and LFS. A recent report provided extensive bioinformatic advice to correctly call this variant from background noise in NGS traces, concluding that ASXL1G646Wfs*12 variant is a bona fide mutation14 ; another study indicated that this variant may represent an early mutational event in a dominant clonal population of hematopoietic cells.15 Finally, it seems reasonable, but remains to be shown, that ASXL1 G646Wfs*12 has similar adverse prognostic relevance in myelodysplastic syndromes and acute leukemia.