In this study we report the characterization of two new aptamers selected against troponin T [a biomarker for the diagnosis of acute myocardial infarction (AMI)] by surface plasmon resonance (SPR), a reference optical platform for label-free detection of molecular binding interactions, and the possible set up of an ELONA assay for TnT detection based on a single detecting aptamer adsorbed on microwell (direct ELONA), or on the couple of aptamers in a sandwich format (sandwich ELONA). The subsequent colorimetric detection is achieved in both cases by using streptavidin-horseradish peroxidase (S-HRP) as signal reporter.

Characterization of troponin T binding aptamers for an innovative enzyme-linked oligonucleotide assay (ELONA) / Francesca Torrini, Pasquale Palladino, Alvaro Brittoli, Veronica Baldoneschi, Maria Minunni, Simona Scarano. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - ELETTRONICO. - 411:(2019), pp. 7709-7716. [10.1007/s00216-019-02014-7]

Characterization of troponin T binding aptamers for an innovative enzyme-linked oligonucleotide assay (ELONA)

TORRINI, FRANCESCA;PALLADINO, PASQUALE;BRITTOLI, ALVARO;BALDONESCHI, VERONICA;Maria Minunni;Simona Scarano
Project Administration
2019

Abstract

In this study we report the characterization of two new aptamers selected against troponin T [a biomarker for the diagnosis of acute myocardial infarction (AMI)] by surface plasmon resonance (SPR), a reference optical platform for label-free detection of molecular binding interactions, and the possible set up of an ELONA assay for TnT detection based on a single detecting aptamer adsorbed on microwell (direct ELONA), or on the couple of aptamers in a sandwich format (sandwich ELONA). The subsequent colorimetric detection is achieved in both cases by using streptavidin-horseradish peroxidase (S-HRP) as signal reporter.
411
7709
7716
Goal 3: Good health and well-being
Francesca Torrini, Pasquale Palladino, Alvaro Brittoli, Veronica Baldoneschi, Maria Minunni, Simona Scarano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1173625
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