Characterization of troponin T binding aptamers for an innovative enzyme-linked oligonucleotide assay (ELONA)

In this study we report the characterization of two new aptamers selected against troponin T [a biomarker for the diagnosis of acute myocardial infarction (AMI)] by surface plasmon resonance (SPR), a reference optical platform for label-free detection of molecular binding interactions, and the possible set up of an ELONA assay for TnT detection based on a single detecting aptamer adsorbed on microwell (direct ELONA), or on the couple of aptamers in a sandwich format (sandwich ELONA). The subsequent colorimetric detection is achieved in both cases by using streptavidin-horseradish peroxidase (S-HRP) as signal reporter.