We studied vaginal microbiota in 46 pregnant women of predominantly Caucasian ethnicity diagnosed with short cervix (<25 mm), and identified microbial communities associated with extreme cervical shortening (≤10 mm). Vaginal microbiota was defined by 16S rRNA gene sequencing and clustered into community state types (CSTs), based on dominance or depletion of Lactobacillus spp. No correlation between CSTs distribution and maternal age or gestational age was revealed. CST-IV, dominated by aerobic and anaerobic bacteria different than Lactobacilli, was associated with extreme cervical shortening (odds ratio (OR) = 15.0, 95% confidence interval (CI) = 1.56–14.21; p = 0.019). CST-III (L. iners-dominated) was also associated with extreme cervical shortening (OR = 6.4, 95% CI = 1.32–31.03; p = 0.02). Gestational diabetes mellitus (GDM) was diagnosed in 10/46 women. Bacterial richness was significantly higher in women experiencing this metabolic disorder, but no association with cervical shortening was revealed by statistical analysis. Our study confirms that Lactobacillus-depleted microbiota is significantly associated with an extremely short cervix in women of predominantly Caucasian ethnicity, and also suggests an association between L. iners-dominated microbiota (CST III) and cervical shortening

Identification of Vaginal Microbial Communities Associated with Extreme Cervical Shortening in Pregnant Women / Monica Di Paola; Viola Seravalli; Sara Paccosi; Carlotta Linari; Astrid Parenti; Carlotta De Filippo; Michele Tanturli; Francesco Vitali; Maria Gabriella Torcia; Mariarosaria Di Tommaso. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - ELETTRONICO. - 9:(2020), pp. 3621-3636. [https://doi.org/10.3390/jcm9113621]

Identification of Vaginal Microbial Communities Associated with Extreme Cervical Shortening in Pregnant Women

Monica Di Paola;Viola Seravalli;Sara Paccosi;Carlotta Linari;Astrid Parenti;Michele Tanturli;Maria Gabriella Torcia
;
Mariarosaria Di Tommaso
2020

Abstract

We studied vaginal microbiota in 46 pregnant women of predominantly Caucasian ethnicity diagnosed with short cervix (<25 mm), and identified microbial communities associated with extreme cervical shortening (≤10 mm). Vaginal microbiota was defined by 16S rRNA gene sequencing and clustered into community state types (CSTs), based on dominance or depletion of Lactobacillus spp. No correlation between CSTs distribution and maternal age or gestational age was revealed. CST-IV, dominated by aerobic and anaerobic bacteria different than Lactobacilli, was associated with extreme cervical shortening (odds ratio (OR) = 15.0, 95% confidence interval (CI) = 1.56–14.21; p = 0.019). CST-III (L. iners-dominated) was also associated with extreme cervical shortening (OR = 6.4, 95% CI = 1.32–31.03; p = 0.02). Gestational diabetes mellitus (GDM) was diagnosed in 10/46 women. Bacterial richness was significantly higher in women experiencing this metabolic disorder, but no association with cervical shortening was revealed by statistical analysis. Our study confirms that Lactobacillus-depleted microbiota is significantly associated with an extremely short cervix in women of predominantly Caucasian ethnicity, and also suggests an association between L. iners-dominated microbiota (CST III) and cervical shortening
9
3621
3636
Goal 3: Good health and well-being
Monica Di Paola; Viola Seravalli; Sara Paccosi; Carlotta Linari; Astrid Parenti; Carlotta De Filippo; Michele Tanturli; Francesco Vitali; Maria Gabriella Torcia; Mariarosaria Di Tommaso
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1216035
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