Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic species (two E.coli strains, three K. pneumoniae strains, P. aeruginosa and E. cloacae) on motility, viability, mitochondrial oxidative status, DNA fragmentation and caspase activity of human spermatozoa. All strains, except P. aeruginosa, reduced significantly sperm motility, with variable effects. Sperm Immobilizing Factor (SIF) was largely responsible for deteriorating effects on sperm motility of E. coli strains since they were completely reverted by knockout of SIF coding recX gene. Sequence alignment for RecX showed the presence of high homologous sequences in K. pneumoniae and E. cloacae but not in P. aeruginosa. These results suggest that, in addition to E.coli, other common uropathogenic Gram-negative bacteria affect sperm motility through RecX product. In addition to sperm motility, the E. coli strain ATCC 35218 also affected sperm viability, and induced caspase activity, oxidative stress and DNA fragmentation suggesting an interspecies variability in the amount and/or type of the produced spermatotoxic factors. In general, our results highlight the need for a careful evaluation of semen infections in the diagnostic process of the infertile man.

Effects of common gram‑negative pathogens causing male genitourinary‑tract infections on human sperm functions / Sara Marchiani, Ilaria Baccani, Lara Tamburrino, Giorgio Mattiuz, Sabrina Nicolò, Chiara Bonaiuto, Carmen Panico, Linda Vignozzi, Alberto Antonelli, Gian maria Rossolini, Maria Torcia, Elisabetta Baldi. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - (2021), pp. 0-0. [10.1038/s41598-021-98710-5]

Effects of common gram‑negative pathogens causing male genitourinary‑tract infections on human sperm functions

Sara Marchiani;Ilaria Baccani;Lara Tamburrino;Giorgio Mattiuz;Sabrina Nicolò;Chiara Bonaiuto;Linda Vignozzi;Alberto Antonelli;Gian maria Rossolini;Maria Torcia;Elisabetta Baldi
2021

Abstract

Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic species (two E.coli strains, three K. pneumoniae strains, P. aeruginosa and E. cloacae) on motility, viability, mitochondrial oxidative status, DNA fragmentation and caspase activity of human spermatozoa. All strains, except P. aeruginosa, reduced significantly sperm motility, with variable effects. Sperm Immobilizing Factor (SIF) was largely responsible for deteriorating effects on sperm motility of E. coli strains since they were completely reverted by knockout of SIF coding recX gene. Sequence alignment for RecX showed the presence of high homologous sequences in K. pneumoniae and E. cloacae but not in P. aeruginosa. These results suggest that, in addition to E.coli, other common uropathogenic Gram-negative bacteria affect sperm motility through RecX product. In addition to sperm motility, the E. coli strain ATCC 35218 also affected sperm viability, and induced caspase activity, oxidative stress and DNA fragmentation suggesting an interspecies variability in the amount and/or type of the produced spermatotoxic factors. In general, our results highlight the need for a careful evaluation of semen infections in the diagnostic process of the infertile man.
2021
0
0
Goal 3: Good health and well-being for people
Sara Marchiani, Ilaria Baccani, Lara Tamburrino, Giorgio Mattiuz, Sabrina Nicolò, Chiara Bonaiuto, Carmen Panico, Linda Vignozzi, Alberto Antonelli, G...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1244074
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