Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human endothelial and vascular smooth muscle cells. In particular, erucin actions were evaluated on cell viability, ROS, caspase 3/7, inflammatory markers levels and the endothelial hyperpermeability in an inflammatory model associated with high glucose concentrations (25 mM, HG). Erucin significantly prevented the HG-induced decrease in cell viability as well as the increase in ROS, caspase 3/7 activation, and TNF-α and IL-6 levels. Similarly, erucin suppressed COX-2 and NF-κB upregulation associated with HG exposure. Erucin also caused a significant inhibition of p22phox subunit expression in endothelial cells. In addition, erucin significantly prevented the HG-induced increase in endothelial permeability as also confirmed by the quantification of the specific markers VE-Cadherin and ZO-1. In conclusion, our results assess anti-inflammatory and antioxidant effects by erucin in vascular cells undergoing HG-induced inflammation and this protection parallels the preservation of endothelial barrier properties.

The H 2 S-Donor Erucin Exhibits Protective Effects against Vascular Inflammation in Human Endothelial and Smooth Muscle Cells / Alma Martelli 1 2 3, Eugenia Piragine 1, Era Gorica 1, Valentina Citi 1, Lara Testai , Eleonora Pagnotta 4, Luca Lazzeri 4, Nicola Pecchioni 5, Valerio Ciccone 6, Rosangela Montanaro 7, Lorenzo Di Cesare Mannelli , Carla Ghelardini , Vincenzo Brancaleone 7, Lucia Morbidelli 6, Vincenzo Calderone. - In: ANTIOXIDANTS. - ISSN 2076-3921. - ELETTRONICO. - (2021), pp. 961-961.

The H 2 S-Donor Erucin Exhibits Protective Effects against Vascular Inflammation in Human Endothelial and Smooth Muscle Cells

Lorenzo Di Cesare Mannelli;Carla Ghelardini;
2021

Abstract

Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human endothelial and vascular smooth muscle cells. In particular, erucin actions were evaluated on cell viability, ROS, caspase 3/7, inflammatory markers levels and the endothelial hyperpermeability in an inflammatory model associated with high glucose concentrations (25 mM, HG). Erucin significantly prevented the HG-induced decrease in cell viability as well as the increase in ROS, caspase 3/7 activation, and TNF-α and IL-6 levels. Similarly, erucin suppressed COX-2 and NF-κB upregulation associated with HG exposure. Erucin also caused a significant inhibition of p22phox subunit expression in endothelial cells. In addition, erucin significantly prevented the HG-induced increase in endothelial permeability as also confirmed by the quantification of the specific markers VE-Cadherin and ZO-1. In conclusion, our results assess anti-inflammatory and antioxidant effects by erucin in vascular cells undergoing HG-induced inflammation and this protection parallels the preservation of endothelial barrier properties.
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Alma Martelli 1 2 3, Eugenia Piragine 1, Era Gorica 1, Valentina Citi 1, Lara Testai , Eleonora Pagnotta 4, Luca Lazzeri 4, Nicola Pecchioni 5, Valerio Ciccone 6, Rosangela Montanaro 7, Lorenzo Di Cesare Mannelli , Carla Ghelardini , Vincenzo Brancaleone 7, Lucia Morbidelli 6, Vincenzo Calderone
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/1260196
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