In rodent motor cortex, the rostral forelimb area (RFA) and the caudal forelimb area (CFA) are major actors in orchestrating the control of forelimb complex movements. However, their intrinsic connections and reciprocal functional organization are still unclear, limiting our understanding of how the brain coordinates and executes voluntary movements. Here we causally probed cortical connectivity and activation patterns triggered by transcranial optogenetic stimulation of ethologically relevant complex movements exploiting a novel large-scale all-optical method in awake mice. Results show specific activation features for each movement class, providing evidence for a segregated functional organization of CFA and RFA. Importantly, we identified a second discrete lateral grasping representation area, namely lateral forelimb area (LFA), with unique connectivity and activation patterns. Therefore, we propose the LFA as a distinct motor representation in the forelimb somatotopic motor map.

Large-scale all-optical dissection of motor cortex connectivity shows a segregated organization of mouse forelimb representations / Francesco Resta, Elena Montagni, Giuseppe de Vito, Alessandro Scaglione, Anna Letizia Allegra Mascaro, Francesco Saverio Pavone. - In: CELL REPORTS. - ISSN 2211-1247. - ELETTRONICO. - 41:(2022), pp. 111627.0-111627.0. [10.1016/j.celrep.2022.111627]

Large-scale all-optical dissection of motor cortex connectivity shows a segregated organization of mouse forelimb representations

Francesco Resta
;
Elena Montagni;Giuseppe de Vito;Alessandro Scaglione;Anna Letizia Allegra Mascaro
;
Francesco Saverio Pavone
2022

Abstract

In rodent motor cortex, the rostral forelimb area (RFA) and the caudal forelimb area (CFA) are major actors in orchestrating the control of forelimb complex movements. However, their intrinsic connections and reciprocal functional organization are still unclear, limiting our understanding of how the brain coordinates and executes voluntary movements. Here we causally probed cortical connectivity and activation patterns triggered by transcranial optogenetic stimulation of ethologically relevant complex movements exploiting a novel large-scale all-optical method in awake mice. Results show specific activation features for each movement class, providing evidence for a segregated functional organization of CFA and RFA. Importantly, we identified a second discrete lateral grasping representation area, namely lateral forelimb area (LFA), with unique connectivity and activation patterns. Therefore, we propose the LFA as a distinct motor representation in the forelimb somatotopic motor map.
2022
41
0
0
Francesco Resta, Elena Montagni, Giuseppe de Vito, Alessandro Scaglione, Anna Letizia Allegra Mascaro, Francesco Saverio Pavone
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1285392
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