Abstract: Oil production waste products (OPWPs) derive from olive mill and represent a crucial environmental problem due to their high polyphenolic content able to pollute the ground. One option to reduce the OPWPs’ environmental impact is to exploit polyphenols’ biological properties. We sought to analyze the transcriptomic variations of colorectal cancer cells exposed to the OPWPs extracts and hydroxytyrosol, the major component, to recognize unknown and ill-defined characteristics. Among the top affected pathways identified by GSEA, we focused on oxidative phosphorylation in an in vitro system. Colorectal cancer HCT116 and LoVo cells treated with hydroxytyrosol or OPWPs extracts showed enhancement of the respiratory chain complexes’ protein levels, ATP production and membrane potential, suggesting stimulation of mitochondrial functions. The major proteins involved in mitochondrial biogenesis and fusion events of mitochondrial dynamics were positively affected, as byWestern blot, fostering increase of the mitochondrial mass organized in a network of elongated organelles. Mechanistically, we proved that PPAR mediates the effects as they are mimicked by a specific ligand and impaired by a specific inhibitor. OPWP extracts and hydroxytyrosol, thus, promote mitochondrial functionality via a feed-forward regulatory loop involving the PPAR /PGC-1a axis. These results support their use in functional foods and as adjuvants in cancer therapy.

Transcriptomic analysis of colorectal cancer cells treated with oil production waste products (OPWPs) reveals enrichment of pathways of mitochondrial functionality / Manuela Leo, Livio Muccillo, Erica Pranzini, Giovannina Barisciano, Matteo Parri, Giulia Lopatriello, Marco Carlomagno, Alice Santi, Maria Letizia Taddei, Lina Sabatino. - In: CELLS. - ISSN 2073-4409. - STAMPA. - 11:(2022), pp. 3992-4006. [10.3390/cells11243992]

Transcriptomic analysis of colorectal cancer cells treated with oil production waste products (OPWPs) reveals enrichment of pathways of mitochondrial functionality

Erica Pranzini;Matteo Parri;Alice Santi;Maria Letizia Taddei;
2022

Abstract

Abstract: Oil production waste products (OPWPs) derive from olive mill and represent a crucial environmental problem due to their high polyphenolic content able to pollute the ground. One option to reduce the OPWPs’ environmental impact is to exploit polyphenols’ biological properties. We sought to analyze the transcriptomic variations of colorectal cancer cells exposed to the OPWPs extracts and hydroxytyrosol, the major component, to recognize unknown and ill-defined characteristics. Among the top affected pathways identified by GSEA, we focused on oxidative phosphorylation in an in vitro system. Colorectal cancer HCT116 and LoVo cells treated with hydroxytyrosol or OPWPs extracts showed enhancement of the respiratory chain complexes’ protein levels, ATP production and membrane potential, suggesting stimulation of mitochondrial functions. The major proteins involved in mitochondrial biogenesis and fusion events of mitochondrial dynamics were positively affected, as byWestern blot, fostering increase of the mitochondrial mass organized in a network of elongated organelles. Mechanistically, we proved that PPAR mediates the effects as they are mimicked by a specific ligand and impaired by a specific inhibitor. OPWP extracts and hydroxytyrosol, thus, promote mitochondrial functionality via a feed-forward regulatory loop involving the PPAR /PGC-1a axis. These results support their use in functional foods and as adjuvants in cancer therapy.
2022
11
3992
4006
Manuela Leo, Livio Muccillo, Erica Pranzini, Giovannina Barisciano, Matteo Parri, Giulia Lopatriello, Marco Carlomagno, Alice Santi, Maria Letizia Taddei, Lina Sabatino
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1293960
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