ICBEB Course NMR for combatting diseases: from cancer to SARS-CoV-2 - My first 1D and 2D NMR spectra: initial set-up, data acquisition and processing

Trainer in the course entitled My first 1D and 2D NMR spectra: initial set-up, data acquisition and processing. The abstract of the curse is reported hereafter. This practical course will be focused on the first fundamental steps and concepts that a spectroscopist must take into account when sitting in front of an NMR instrument to properly acquire and process the first, fundamental, spectra. These spectra will represent the fingerprint of a protein in solution and can provide plenty of information with atomic resolution. The practical will start with the standard procedures needed to set up the optimal instrumental conditions for the acquisition of NMR spectra such as the choice of temperature, the tuning and matching process and the shimming routine. Examples of bad Vs optimal shimming conditions will be provided. The importance of setting the carrier in an accurate position will be shown together with the effect on the calibration of 1H hard pulses. The demonstration of possible ways to properly calibrate hard pulses will be shown eventually on different nuclei. A series of monodimensional 1H-detected experiments exploiting different solvent suppression schemes will be described and acquired pointing the attention also to the key pulse program parameters. The main principles of bidimensional experiments will be described and a few examples of 2D experiments will be acquired. A brief description of the information that can be grasped from different kinds of 2D spectra will be provided. During the practical, the basic processing procedures will be illustrated mainly focusing on the phasing procedure and different processing parameters.