Expression and Biophysical Characterization of Therapeutically-Relevant Proteins: Insights into Siglecs and Beyond

This research successfully demonstrated the expression, structural characterization, and functional analysis of multiple immunomodulatory and therapeutic proteins. These findings contribute to the broader understanding of protein-ligand interactions, glycomimetic therapeutics, and novel delivery systems, with particular emphasis on immune checkpoint targets and biologics encapsulated in red blood cells. This dual approach for studying both immunologically significant proteins and biologics in drug delivery systems underscores the versatility and applicability of the methods used in this work. By integrating structural characterization techniques with functional analyses, this study not only advances our understanding of protein-ligand interactions and glycomimetic-based therapeutics, but also highlights the potential of red blood cells as efficient carriers for biologics. Together, these findings pave the way for future advancements in both therapeutic development and biologically-relevant delivery technologies.