This study investigates the role of the GLRX3-BOLA2 complex in the cytosolic assembly of [4Fe-4S] clusters and the maturation of NUBP1. Using a combination of spectroscopic and biochemical approaches, the researchers monitored Fe-S cluster formation and stability. UV–vis spectroscopy confirmed cluster integrity, while paramagnetic NMR provided direct evidence for [4Fe-4S] cluster formation on NUBP1. Anaerobic reconstitution assays further evaluated the efficiency of cluster assembly under controlled redox conditions. Together, these results shed light on the stepwise mechanism of [4Fe-4S] cluster biogenesis in human cells.
BOLA2 and GLRX3: keyplayers in human cytosolic [4Fe-4S] cluster assembly / Martina Masini, Francesca Camponeschi, Lucia Banci. - ELETTRONICO. - (2025), pp. 0-0. ( 15th European Biophysics Congress Roma ).
BOLA2 and GLRX3: keyplayers in human cytosolic [4Fe-4S] cluster assembly
Martina Masini;Francesca Camponeschi;Lucia Banci
2025
Abstract
This study investigates the role of the GLRX3-BOLA2 complex in the cytosolic assembly of [4Fe-4S] clusters and the maturation of NUBP1. Using a combination of spectroscopic and biochemical approaches, the researchers monitored Fe-S cluster formation and stability. UV–vis spectroscopy confirmed cluster integrity, while paramagnetic NMR provided direct evidence for [4Fe-4S] cluster formation on NUBP1. Anaerobic reconstitution assays further evaluated the efficiency of cluster assembly under controlled redox conditions. Together, these results shed light on the stepwise mechanism of [4Fe-4S] cluster biogenesis in human cells.
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