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|Titolo:||Coenzyme Q10 prevents adipocyte apoptosis following collection for lipofilling|
|Autori interni:||WITORT, EWA JANINA|
LO RUSSO, GIULIA
LO RUSSO, DOMENICO
|Data di pubblicazione:||2004|
|Rivista:||RIVISTA ITALIANA DI CHIRURGIA PLASTICA|
|Abstract:||We have previously observed that Coenzyme Q10 the well-known component of the respiratory chain as well as scavenger of free radicals, inhibits apoptotic cellular death of cultured corneal keratocytes following treatment with excimer laser, a treatment inducing apoptosis through free radicals formation. This phenomenon has also been confirmed in vivo. Consequently, we have also demonstrated that Coenzyme Q10 protects against apoptotic death induced by non free radicals producing agents and therefore, independently its function of antioxidant molecule. Considering a possible application of Coenzyme Q10 as an anti apoptotic agent in plastic surgery, we have examined its ability to protect from apoptotic death human adipocytes collected for llipofilling. Adipocytes were collected with a Luer-lock syringe according to Coleman's technique or by means of liposuctor with -680 mmHg vacuum. Following collection, half of each sample was treated with 10μM Coenzyme Q10 dissolved in Lutrol and physiological salts solution, and the other half was treated with Lutrol and physiological salts solution only. The adipocytes were then processed according to the Coleman's technique and preserved in liquid nitrogen. Apotosis was quantified on the basis of detection cytochrome c that is released from mitochondrion to cytoplasm following apoptotic stimulus or by morphological analysis of adipocytes stained with Hoechst 33258 fluorescent dye that allows detection of structural changes and fragmentation of nucleus in apoptotic cells by means of fluorescent microscope with attached digital photo camera. The levels of cytochrome c in cytoplasm of adipocytes collected with liposuctor were significantly higher then in adipocytes collected by Coleman's method. Moreover, presence of Coenzyme Q10 during preoperative stage consisting of centrifugation and purification of adipocytes further reduced levels of cytochrome c released to cytoplasm and therefore apoptotic death. In agreement with results of biochemical analysis, correspnding samples treaed with Coenzyme Q10 and analysed by fluorescent microscopy demonstrate significantly reduced number of cells with apoptotic morphology as compared to non treated controls. Finally, our working hypothesis that Coenzyme Q10 exerts strong anti apoptotic activity against stress to which adipocytes are subjecte during all phases of preparation preceding lipofilling has been confirmed.|
|Appare nelle tipologie:||1a - Articolo su rivista|
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