We investigated the infection of peripheral blood mononuclear cells (PBMNC) by hepatitis C virus (HCV) in 5 patients with HCV-related chronic hepatitis. The presence of HCV-RNA-positive and -negative strands was tested with the polymerase chain reaction (PCR) method. In all subjects, HCV-RNA was shown in PBMNC. In 3 cases, HCV-RNA was shown in the T- and B-cell populations, with viral RNA also present in the monocyte-macrophage fraction of two of these. HCV-RNA-negative stranded molecules, indicative of the viral multiplication, were significantly increased in cells maintained in cultures with PHA/PMA stimulation. The results indicate that HCV infect blood mononuclear cells, thus suggesting that this cellular tropism may play a role in HCV infection.

Infection of peripheral mononuclear blood cells by hepatitis C virus / A.L. Zignego; D. Macchia; M. Monti; V. Thiers; M. Mazzetti; M. Foschi; E. Maggi; S. Romagnani; P. Gentilini; C. Brechot. - In: JOURNAL OF HEPATOLOGY. - ISSN 0168-8278. - STAMPA. - 15:(1992), pp. 382-386.

Infection of peripheral mononuclear blood cells by hepatitis C virus

ZIGNEGO, ANNA LINDA;MONTI, MONICA;FOSCHI, MARCO;MAGGI, ENRICO;ROMAGNANI, SERGIO;GENTILINI, PAOLO;
1992

Abstract

We investigated the infection of peripheral blood mononuclear cells (PBMNC) by hepatitis C virus (HCV) in 5 patients with HCV-related chronic hepatitis. The presence of HCV-RNA-positive and -negative strands was tested with the polymerase chain reaction (PCR) method. In all subjects, HCV-RNA was shown in PBMNC. In 3 cases, HCV-RNA was shown in the T- and B-cell populations, with viral RNA also present in the monocyte-macrophage fraction of two of these. HCV-RNA-negative stranded molecules, indicative of the viral multiplication, were significantly increased in cells maintained in cultures with PHA/PMA stimulation. The results indicate that HCV infect blood mononuclear cells, thus suggesting that this cellular tropism may play a role in HCV infection.
1992
15
382
386
A.L. Zignego; D. Macchia; M. Monti; V. Thiers; M. Mazzetti; M. Foschi; E. Maggi; S. Romagnani; P. Gentilini; C. Brechot
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/226195
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