This study was designed to develop efficient analytical tools for the difficult HPLC-DAD-MS identification of hydrolyzable tannins in natural tissue extracts. Throughout the study of the spectroscopic characteristics of properly synthesized stereodefined standards, it was observed that the UV-vis spectra of compounds with the m-depsidic link showed a characteristic shoulder at 300 nm, consistent with the simple glucogalloyl esters, whereas compounds with the hexahydroxydiphenoyl (HHDP) unit gave a diagnostic fragmentation pattern, caused by a spontaneous lactonization in the mass spectrometer. These observations were confirmed by HPLC-DAD-MS analyses of tannic acid and raspberry extracts, which are rich in hydrolyzable tannins with them-depsidic link and the HHDP unit, respectively
Tannins with the Hexahydroxydiphenoyl Unit and the m-Depsidic Link: HPLC-DAD-MS Identification and Model Synthesis / P. Arapitsas; S. Menichetti; F.F. Vincieri; A. Romani. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - STAMPA. - 55:(2007), pp. 48-55. [10.1021/jf0622329]
Tannins with the Hexahydroxydiphenoyl Unit and the m-Depsidic Link: HPLC-DAD-MS Identification and Model Synthesis
MENICHETTI, STEFANO;VINCIERI, FRANCO FRANCESCO;ROMANI, ANNALISA
2007
Abstract
This study was designed to develop efficient analytical tools for the difficult HPLC-DAD-MS identification of hydrolyzable tannins in natural tissue extracts. Throughout the study of the spectroscopic characteristics of properly synthesized stereodefined standards, it was observed that the UV-vis spectra of compounds with the m-depsidic link showed a characteristic shoulder at 300 nm, consistent with the simple glucogalloyl esters, whereas compounds with the hexahydroxydiphenoyl (HHDP) unit gave a diagnostic fragmentation pattern, caused by a spontaneous lactonization in the mass spectrometer. These observations were confirmed by HPLC-DAD-MS analyses of tannic acid and raspberry extracts, which are rich in hydrolyzable tannins with them-depsidic link and the HHDP unit, respectivelyFile | Dimensione | Formato | |
---|---|---|---|
2007_JAFC_07_48.pdf
Accesso chiuso
Descrizione: Articolo principale
Tipologia:
Pdf editoriale (Version of record)
Licenza:
Tutti i diritti riservati
Dimensione
328.4 kB
Formato
Adobe PDF
|
328.4 kB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.