In recent years carbon monoxide (CO) has been employed by the fishery industry to preserve the fresh appearance of fish products during frozen storage, particularly in vacuum packaged products. CO reacts with oxy-myoglobin to form a stable cherry coloured carboxy-myoglobin complex. We propose a method that allows the quantitative determination of CO in the meat drip of tuna fish by UV–Vis spectroscopy. It consists of: (i) preparations of CO secondary standard solutions titrated against horse heart Mb primary standard solutions; (ii) determination of calibration curves to measure CO concentration in treated and untreated samples. The accuracy of the spectrophotometric method has been evaluated in terms of trueness and precision by using fortified tuna fish samples. The spectroscopic results have been compared with those obtained using a head space gas chromatographic technique (HS-GC–MS). The CO levels measured in tuna fish samples by UV–Vis are substantially lower than those revealed by HS-GC–MS. The origin of this discrepancy is discussed.

Development and validation of a quantitative spectrophotometric method to detect the amount of carbon monoxide in treated tuna fish / Enrica Droghetti; Gian Luca Bartolucci; Claudia Focardi; Massimo Bambagiotti-Alberti; Mila Nocentini; Giulietta Smulevich. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - STAMPA. - 128:(2011), pp. 1143-1151. [10.1016/j.foodchem.2011.04.002]

Development and validation of a quantitative spectrophotometric method to detect the amount of carbon monoxide in treated tuna fish.

DROGHETTI, ENRICA;BARTOLUCCI, GIAN LUCA;Massimo Bambagiotti Alberti;SMULEVICH, GIULIETTA
2011

Abstract

In recent years carbon monoxide (CO) has been employed by the fishery industry to preserve the fresh appearance of fish products during frozen storage, particularly in vacuum packaged products. CO reacts with oxy-myoglobin to form a stable cherry coloured carboxy-myoglobin complex. We propose a method that allows the quantitative determination of CO in the meat drip of tuna fish by UV–Vis spectroscopy. It consists of: (i) preparations of CO secondary standard solutions titrated against horse heart Mb primary standard solutions; (ii) determination of calibration curves to measure CO concentration in treated and untreated samples. The accuracy of the spectrophotometric method has been evaluated in terms of trueness and precision by using fortified tuna fish samples. The spectroscopic results have been compared with those obtained using a head space gas chromatographic technique (HS-GC–MS). The CO levels measured in tuna fish samples by UV–Vis are substantially lower than those revealed by HS-GC–MS. The origin of this discrepancy is discussed.
2011
128
1143
1151
Enrica Droghetti; Gian Luca Bartolucci; Claudia Focardi; Massimo Bambagiotti-Alberti; Mila Nocentini; Giulietta Smulevich
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/544465
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