Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMP). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO(2)(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO(2)-(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse.

Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy / S. Nappini;T. A. Kayal;D. Berti;B. Norden;P. Baglioni. - In: THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS. - ISSN 1948-7185. - STAMPA. - 2:(2011), pp. 713-718. [10.1021/jz2000936]

Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

NAPPINI, SILVIA;BERTI, DEBORA;BAGLIONI, PIERO
2011

Abstract

Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMP). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO(2)(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO(2)-(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse.
2011
2
713
718
S. Nappini;T. A. Kayal;D. Berti;B. Norden;P. Baglioni
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/593929
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