The exact phenotype and lineage of endothelial progenitor cells (EPCs) are still a matter of debate and different expansion protocols are used to obtain them. In this study, EPC expansion from peripheral blood mononuclear cells was analyzed within the first week of culture. Both the adherent and suspended cells, of which the latter usually discarded, were considered. We provide, for the first time, a systematic study of EPC phenotype and functional features within the first 3 days of culture. Moreover, within the 2nd day, both cellular fractions displayed a significant increase in endothelial marker expression which correlated with EPC properties.
Human Endothelial Progenitor Cells / Lydia Bellik; Maria Cristina Vinci; Fabrizio Ledda; Parenti A. - In: JOURNAL OF VASCULAR RESEARCH. - ISSN 1018-1172. - STAMPA. - 42:(2005), pp. II/1-II/1. (Intervento presentato al convegno 3rd European Meeting on Vascular Biology and Medicine tenutosi a Hamburg, Germany nel September 28-30, 2005).
Human Endothelial Progenitor Cells
LEDDA, FABRIZIO;PARENTI, ASTRID
2005
Abstract
The exact phenotype and lineage of endothelial progenitor cells (EPCs) are still a matter of debate and different expansion protocols are used to obtain them. In this study, EPC expansion from peripheral blood mononuclear cells was analyzed within the first week of culture. Both the adherent and suspended cells, of which the latter usually discarded, were considered. We provide, for the first time, a systematic study of EPC phenotype and functional features within the first 3 days of culture. Moreover, within the 2nd day, both cellular fractions displayed a significant increase in endothelial marker expression which correlated with EPC properties.
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