The exposure to microgravity conditions results in a significant impairment of the immune function. Many reports describe morphological and functional changes in T-lymphocytes, monocytes and neutrophil granulocytes cultured in microgravity, both real and modeled, but very few studies have been made on the effect of microgravity on dendritic cells (DCs) and DC differentiation. DO are able to process antigens and are the most efficient cells in presenting them to T-lymphocytes, thus giving a crucial contribution to the rise of an effective immune response. The aim of this study was to investigate whether the maturation of DO,from monocytes of astronauts was altered postflight. Blood samples from a crew-member of the Eneide mission were collected before the flight, soon after the return to earth and one year after the mission. In order to generate DO, monocytes were used as precursors. The), were separated, cultured for 6 days in medium supplemented with granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 4 (IL-4), then furtherly stimulated for 24 hours with a cocktail of cytokines. Differentiation was assessed by flow cytometry and immunofluorescence, assaying the expression of typical DC markers. Gene expression was analysed by RT-PCR. Morphological and junctional characteristics were studied by autofluorescence microscopy. The findings showed that the maturation of DO from inonocytes collected from the astronaut immediately postflight was altered. In comparison with controls, significant differences were found in expression of DC markers, expression of genes involved in DC maturation, morphological and junctional characteristics.

Does the exposure to microgravity affect dendritic cell maturation from manocytes? / Monici M; Basile V; Bellik L; Fusi F; Marziliano N; Parenti A; Romano G; Conti A. - STAMPA. - (2006), pp. ---. (Intervento presentato al convegno Science on European Soyuz Missions to the International Space Station (2001-2005) tenutosi a Toledo (Spain) nel 27th -30th June 2006.).

Does the exposure to microgravity affect dendritic cell maturation from manocytes?

MONICI, MONICA;FUSI, FRANCO;PARENTI, ASTRID;ROMANO, GIOVANNI;CONTI, ANTONIO
2006

Abstract

The exposure to microgravity conditions results in a significant impairment of the immune function. Many reports describe morphological and functional changes in T-lymphocytes, monocytes and neutrophil granulocytes cultured in microgravity, both real and modeled, but very few studies have been made on the effect of microgravity on dendritic cells (DCs) and DC differentiation. DO are able to process antigens and are the most efficient cells in presenting them to T-lymphocytes, thus giving a crucial contribution to the rise of an effective immune response. The aim of this study was to investigate whether the maturation of DO,from monocytes of astronauts was altered postflight. Blood samples from a crew-member of the Eneide mission were collected before the flight, soon after the return to earth and one year after the mission. In order to generate DO, monocytes were used as precursors. The), were separated, cultured for 6 days in medium supplemented with granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 4 (IL-4), then furtherly stimulated for 24 hours with a cocktail of cytokines. Differentiation was assessed by flow cytometry and immunofluorescence, assaying the expression of typical DC markers. Gene expression was analysed by RT-PCR. Morphological and junctional characteristics were studied by autofluorescence microscopy. The findings showed that the maturation of DO from inonocytes collected from the astronaut immediately postflight was altered. In comparison with controls, significant differences were found in expression of DC markers, expression of genes involved in DC maturation, morphological and junctional characteristics.
2006
-
Science on European Soyuz Missions to the International Space Station (2001-2005)
Toledo (Spain)
Monici M; Basile V; Bellik L; Fusi F; Marziliano N; Parenti A; Romano G; Conti A
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/676449
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