Dendritic cells (DCs), the most potent antigen-presenting cells inducing specific immune responses, are involved in the pathogenesis of atherosclerosis. In this inflammatory disease, DCs increase in number being particularly abundant in the shoulder regions of plaques (1). Since the exposure to altered gravitational conditions results in a significant impairment of the immune function, the aim of this study was to investigate the effects of hypergravity on the function of DCs and their interactions with the vascular wall cells. Monocytes from peripheral blood mononuclear cells were sorted by CD14+ magnetic beads selection, cultured for 6 days in medium supplemented with GM-CSF and IL-4 followed by a further maturation stimuli (2). DC phenotype, assessed by flow cytometry, showed a high expression of the specific DC markers CD80, CD86, HLA-DR and CD83. The DCs obtained were then exposed to hypergravitational stimuli and their phenotype, cytoskeleton, ability to activate lymphocytes and interaction with vascular wall cells were investigated. The findings showed that the exposure to hypergravity conditions resulted in a significant impairment of DC cytoskeletal organization without affecting the expression of DC markers. Moreover an increased DC adhesion to human vascular smooth muscle cells as well as a change in their ability to activate lymphocytes was observed.

Hypergravity effects on dendritic cell and vascular wall interactions. From Galileo to low gravity / Bellik L; Parenti A; Ledda F; Basile V; Romano G; Fusi F; Monici M. - STAMPA. - 25:(2007), pp. 110-110. (Intervento presentato al convegno ELGRA Biennal Symposium jointly with XX National Meeting of the Italian Association for Aeronautical and Space Medicine tenutosi a Florence nel 4-7 September 2007).

Hypergravity effects on dendritic cell and vascular wall interactions. From Galileo to low gravity

PARENTI, ASTRID;LEDDA, FABRIZIO;ROMANO, GIOVANNI;FUSI, FRANCO;MONICI, MONICA
2007

Abstract

Dendritic cells (DCs), the most potent antigen-presenting cells inducing specific immune responses, are involved in the pathogenesis of atherosclerosis. In this inflammatory disease, DCs increase in number being particularly abundant in the shoulder regions of plaques (1). Since the exposure to altered gravitational conditions results in a significant impairment of the immune function, the aim of this study was to investigate the effects of hypergravity on the function of DCs and their interactions with the vascular wall cells. Monocytes from peripheral blood mononuclear cells were sorted by CD14+ magnetic beads selection, cultured for 6 days in medium supplemented with GM-CSF and IL-4 followed by a further maturation stimuli (2). DC phenotype, assessed by flow cytometry, showed a high expression of the specific DC markers CD80, CD86, HLA-DR and CD83. The DCs obtained were then exposed to hypergravitational stimuli and their phenotype, cytoskeleton, ability to activate lymphocytes and interaction with vascular wall cells were investigated. The findings showed that the exposure to hypergravity conditions resulted in a significant impairment of DC cytoskeletal organization without affecting the expression of DC markers. Moreover an increased DC adhesion to human vascular smooth muscle cells as well as a change in their ability to activate lymphocytes was observed.
2007
bulletin of the european low gravity research association
ELGRA Biennal Symposium jointly with XX National Meeting of the Italian Association for Aeronautical and Space Medicine
Florence
Bellik L; Parenti A; Ledda F; Basile V; Romano G; Fusi F; Monici M
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/676455
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