The efficiency of two-dimensional homonuclear 1H-1H correlated spectroscopy and two-dimensional reverse heteronuclear shift correlation spectroscopy (i.e., heteronuclear multiple quantum correlation) in characterizing and evaluating the relative content of herbal extract constituents is demonstrated. These experiments are able to fully assign the proton and carbon resonances of all three classes of constituents present in dried commercial extract of St. John’s wort, that is, flavonols, phloroglucinols, and naphthodianthrones, with particular regard to the very unstable phloroglucinols. In addition, shikimic and chlorogenic acids, sucrose, lipids, polyphenols, and traces of solvents of the extractive process (methanol) were also identified. These experiments can be considered to be a very simple and fast analytical method for determining the quality and stability of the titled commercial extract. They represent a generally applicable technique for a rapid screening and a specific measurement of other commercial phytochemicals or, in selected cases, an alternative to the classical analytical techniques such as high-performance thin-layer chromatography, high-performance liquid chromatography, capillary gas chromatography, and electrophoresis.

Analysis of plant complex matrices by use of nuclear magnetic resonance spectroscopy: St. John's wort extract / A. R. Bilia; M. C. Bergonzi; G. Mazzi; F. F. Vincieri. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - ELETTRONICO. - (2001), pp. 2115-2124.

Analysis of plant complex matrices by use of nuclear magnetic resonance spectroscopy: St. John's wort extract.

BILIA, ANNA RITA;BERGONZI, MARIA CAMILLA;MAZZI, GIOVANNI;VINCIERI, FRANCO FRANCESCO
2001

Abstract

The efficiency of two-dimensional homonuclear 1H-1H correlated spectroscopy and two-dimensional reverse heteronuclear shift correlation spectroscopy (i.e., heteronuclear multiple quantum correlation) in characterizing and evaluating the relative content of herbal extract constituents is demonstrated. These experiments are able to fully assign the proton and carbon resonances of all three classes of constituents present in dried commercial extract of St. John’s wort, that is, flavonols, phloroglucinols, and naphthodianthrones, with particular regard to the very unstable phloroglucinols. In addition, shikimic and chlorogenic acids, sucrose, lipids, polyphenols, and traces of solvents of the extractive process (methanol) were also identified. These experiments can be considered to be a very simple and fast analytical method for determining the quality and stability of the titled commercial extract. They represent a generally applicable technique for a rapid screening and a specific measurement of other commercial phytochemicals or, in selected cases, an alternative to the classical analytical techniques such as high-performance thin-layer chromatography, high-performance liquid chromatography, capillary gas chromatography, and electrophoresis.
2001
2115
2124
A. R. Bilia; M. C. Bergonzi; G. Mazzi; F. F. Vincieri
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/775754
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